The major objectives of Program 2 of this overall research program are to prepare extracts of all collected plant materials for initial biological testing and to isolate and structurally characterize novel antineoplastic agents from plants. Species collected in the field will be received by Program 1, and a non-polar (organic solvent-soluble) and a polar (aqueous-soluble) extract will be prepared for each sample. Each non-polar extract will be distributed to Programs 3, 4, and 5 for initial biological evaluation while the aqueous extracts will be stored in a frozen repository in case of later need. Dereplication of known active compounds will be performed by HPLC/MS, coupled with a consideration of prior chemotaxonomic literature. The active principle(s) of each recollected, satisfactorily dereplicated, confirmed-active plant part made available by Program 1 will be isolated by a combination of standard solvent partition and chromatographic procedures, using one of the various bioassays that have been developed by the consortium to guide the fractionation. Spectroscopic and chemical methods will be used to determine the structures of each pure active isolate. Particular emphasis will be given to modern one- and two dimensional nuclear magnetic resonance techniques for compound structure elucidation. To permit more advanced biological testing to be performed and also to aid in analog development studies, active compounds will bed re-isolated in larger quantities, when deemed appropriate by the consortial group. It is anticipated that the overall outcome of Program 2 will be the discovery of one or more selectively active natural products that show promise for future development as cancer chemotherapeutic agents.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program--Cooperative Agreements (U19)
Project #
2U19CA052956-06
Application #
3731075
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1995
Total Cost
Indirect Cost
Name
University of Illinois at Chicago
Department
Type
DUNS #
121911077
City
Chicago
State
IL
Country
United States
Zip Code
60612
Li, Jie; Pan, Li; Deng, Ye et al. (2013) Sphenostylisins A-K: bioactive modified isoflavonoid constituents of the root bark of Sphenostylis marginata ssp. erecta. J Org Chem 78:10166-77
Ren, Yulin; Acuña, Ulyana Muñoz; Jiménez, Francisco et al. (2012) Cytotoxic and NF-?B inhibitory sesquiterpene lactones from Piptocoma rufescens. Tetrahedron 68:2671-2678
Pan, Li; Chai, Hee-Byung; Kinghorn, Alan Douglas (2012) Discovery of new anticancer agents from higher plants. Front Biosci (Schol Ed) 4:142-56
Pan, Li; Yong, Yeonjoong; Deng, Ye et al. (2012) Isolation, structure elucidation, and biological evaluation of 16,23-epoxycucurbitacin constituents from Eleaocarpus chinensis. J Nat Prod 75:444-52
Ren, Yulin; Matthew, Susan; Lantvit, Daniel D et al. (2011) Cytotoxic and NF-?B inhibitory constituents of the stems of Cratoxylum cochinchinense and their semisynthetic analogues. J Nat Prod 74:1117-25
Kinghorn, A Douglas; Pan, Li; Fletcher, Joshua N et al. (2011) The relevance of higher plants in lead compound discovery programs. J Nat Prod 74:1539-55
Deng, Ye; Chin, Young-Won; Chai, Hee-Byung et al. (2011) Phytochemical and Bioactivity Studies on Constituents of the Leaves of Vitex Quinata. Phytochem Lett 4:213-217
Gupta, Sneha V; Sass, Ellen J; Davis, Melanie E et al. (2011) Resistance to the translation initiation inhibitor silvestrol is mediated by ABCB1/P-glycoprotein overexpression in acute lymphoblastic leukemia cells. AAPS J 13:357-64
Pan, Li; Chai, Heebyung; Kinghorn, A Douglas (2010) The continuing search for antitumor agents from higher plants. Phytochem Lett 3:1-8
Pan, Li; Lantvit, Daniel D; Riswan, Soedarsono et al. (2010) Bioactivity-guided isolation of cytotoxic sesquiterpenes of Rolandra fruticosa. Phytochemistry 71:635-40

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