Scientific Core B is the analytical core responsible for cocaine and antibody quantitations in a variety of specimens from animals and humans. The samples analyzed in Scientific Core B will come from Projects 2, 3, and 4.
The specific aims of the Core are as follows: . Development of methods to effectively stabilize cocaine in biological samples including blood, cerebral spinal fluid (CSF), urine, saliva and tissue. Preliminary data for CSF is reported. . Development of a sensitive method to quantitate cocaine and its monoester metabolites after extraction from serum, CSF, urine, saliva or tissue. Preliminary data is reported. . Development of a method to separate free cocaine from antibody-or protein-bound cocaine. . Validation and optimization of methods to quantitate the monoclonal anti-cocaine antibody in serum following injection into rats or humans. . Validation and optimization of an anti-idiotype assay to detect the formation of antibodies to the human monoclonal anti-cocaine antibody when the project reaches the stage where multiple doses of antibody are administered to humans. . Preparation of standard operating procedures and application of appropriate quality control and quality assurance procedures to all analyses to ensure that valid data is produced. . Application of good laboratory practice procedures to all of the analyses performed. Preliminary data on stabilization of CSF samples and measurement of ng/mL concentrations of cocaine have been obtained. The major strengths of the investigators in this core are in analysis and the application of quality control and quality assurance procedures to analysis. Further quality assurance of data will be provided by Br. Buncher who will oversee acquisition of all data.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19DA012043-04
Application #
6464627
Study Section
Project Start
2001-07-01
Project End
2003-06-30
Budget Start
Budget End
Support Year
4
Fiscal Year
2001
Total Cost
Indirect Cost
Name
University of Cincinnati
Department
Type
DUNS #
City
Cincinnati
State
OH
Country
United States
Zip Code
45221
Norman, Andrew B; Tabet, Michael R; Norman, Mantana K et al. (2007) A chimeric human/murine anticocaine monoclonal antibody inhibits the distribution of cocaine to the brain in mice. J Pharmacol Exp Ther 320:145-53
Norman, Andrew B; Tsibulsky, Vladimir L (2006) The compulsion zone: a pharmacological theory of acquired cocaine self-administration. Brain Res 1116:143-52
Tsibulsky, Vladimir L; Norman, Andrew B (2005) Real time computation of in vivo drug levels during drug self-administration experiments. Brain Res Brain Res Protoc 15:38-45
Paula, Stefan; Tabet, Michael R; Farr, Carol D et al. (2004) Three-dimensional quantitative structure-activity relationship modeling of cocaine binding by a novel human monoclonal antibody. J Med Chem 47:133-42
Norman, Andrew B; Buesing, William R; Norman, Mantana K et al. (2004) The self-administration of WIN 35,428 and cocaine: comparisons of satiety threshold and elimination half-life in rats. Eur J Pharmacol 483:281-7
Cabovska, B; Norman, A B; Stalcup, A M (2003) Separation of cocaine stereoisomers by capillary electrophoresis using sulfated cyclodextrins. Anal Bioanal Chem 376:134-7
Paula, Stefan; Tabet, Michael R; Keenan, Susan M et al. (2003) Three-dimensional structure-activity relationship modeling of cocaine binding to two monoclonal antibodies by comparative molecular field analysis. J Mol Biol 325:515-30
Norman, Andrew B; Welge, Jeffrey A; Tsibulsky, Vladimir L (2002) Characterization of the distribution of the cocaine priming threshold and the effect of SCH23390. Brain Res 946:253-61
Norman, A B; Tsibulsky, V L (2001) Satiety threshold regulates maintained self-administration: comment on Lynch and Carroll (2001). Exp Clin Psychopharmacol 9:151-4; discussion 160-2
Tsibulsky, V L; Norma, A B (2001) Satiety threshold during maintained cocaine self-administration in outbred mice. Neuroreport 12:325-8

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