Insights from Studies on Ambient Particulate Matter Engineered nanomaterials (ENMs) are now being developed for a wide range of applications, used in engineering, medicine, sports goods and the leisure industry, and for domestic use such as in cosmetics, clothing, and food. Any biological effects of these novel materials might be expected to reflect their chemistry. However, because the nano-sized particles are transitional, between the atomic molecular components and that of the bulk material, they exhibit extraordinary properties which make it difficult to predict their reactivity simply on the basis of their chemistry. Thus, unique features that make ENMs very attractive for use in novel applications, may also have adverse health effects. There are increasing concerns from prestigious societies (1), government bodies, academic institutes and other organisations, that these new products will result in adverse effects in biological systems (2-5). Initial concern stemmed from epidemiological studies showing that increased cardiorespiratory morbidity and mortality during episodes of air pollution (5-8,13), correlated with increases in the numbers of nanosized particles, particularly diesel exhaust, in the atmosphere. Those with existing lung disease, such as asthma, chronic obstructive pulmonary disease and cancer, were most susceptible. Only a small increase (10 micrograms/meter3) in the amount of ambient particulate matter less that 10 microns (PMio) or 2.5 microns (PM2.5) aerodynamic diameter is needed to increase the risk of myocardial infarct. Other cardiovascular effects include reduced heart rate variability, raised blood pressure and atherosclerosis. Whilst one might anticipate that inhalation of particulate air pollution might have adverse effects on the lung, the cardiovascular effects are surprising and suggest that events taking place within the pulmonary system can cascade into other body systems. These studies highlight the critical role of the lung defence mechanisms in protection from inhaled toxicants. They also highlight the importance ofthe lung as a target organ for atmospheric particulate pollution and the need to understand the mechanisms involved in breaching the pulmonary defence system, in order to manage any risks and hazards of atmospheric exposure to ENM.

National Institute of Health (NIH)
National Institute of Environmental Health Sciences (NIEHS)
Research Program--Cooperative Agreements (U19)
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Special Emphasis Panel (ZES1-SET-V (03))
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University of Southern California
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Botelho, Danielle; Leo, Bey F; Massa, Christopher et al. (2018) Exposure to Silver Nanospheres Leads to Altered Respiratory Mechanics and Delayed Immune Response in an in Vivo Murine Model. Front Pharmacol 9:213
Chung, Kian Fan; Seiffert, Joanna; Chen, Shu et al. (2017) Inactivation, Clearance, and Functional Effects of Lung-Instilled Short and Long Silver Nanowires in Rats. ACS Nano 11:2652-2664
Theodorou, Ioannis G; Ruenraroengsak, Pakatip; Gow, Andrew et al. (2016) Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells. Nanotoxicology 10:1351-62
Seiffert, Joanna; Buckley, Alison; Leo, Bey et al. (2016) Pulmonary effects of inhalation of spark-generated silver nanoparticles in Brown-Norway and Sprague-Dawley rats. Respir Res 17:85
Ruenraroengsak, Pakatip; Chen, Shu; Hu, Sheng et al. (2016) Translocation of Functionalized Multi-Walled Carbon Nanotubes across Human Pulmonary Alveolar Epithelium: Dominant Role of Epithelial Type 1 Cells. ACS Nano 10:5070-85
Chen, S; Goode, A E; Skepper, J N et al. (2016) Avoiding artefacts during electron microscopy of silver nanomaterials exposed to biological environments. J Microsc 261:157-66
Botelho, Danielle J; Leo, Bey Fen; Massa, Christopher B et al. (2016) Low-dose AgNPs reduce lung mechanical function and innate immune defense in the absence of cellular toxicity. Nanotoxicology 10:118-27
Govada, Lata; Leese, Hannah S; Saridakis, Emmanuel et al. (2016) Exploring Carbon Nanomaterial Diversity for Nucleation of Protein Crystals. Sci Rep 6:20053
Seiffert, Joanna; Hussain, Farhana; Wiegman, Coen et al. (2015) Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. PLoS One 10:e0119726
Sarkar, Srijata; Leo, Bey Fen; Carranza, Claudia et al. (2015) Modulation of Human Macrophage Responses to Mycobacterium tuberculosis by Silver Nanoparticles of Different Size and Surface Modification. PLoS One 10:e0143077

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