The UMass Mouse Metabolic Phenotyping Center (MMPC) involves a multidisciplinary group of investigators at UMass who perform an array of novel and sophisticated metabolic, analytical and functional experiments using state-of-the-art equipment for the purpose of investigating transgenic mice useful for understanding obesity, diabetes and its complications. The goal of the UMass MMPC is to provide comprehensive metabolic and functional characterization of transgenic mice using unique and standardized techniques mostly involving in vivo and physiological setting that are complemented by analytical experiments using serum/tissue samples and measure of islet and cardiovascular functions in mice. The UMass MMPC consists of 2 Phenotyping Cores. The Metabolism Core applies hyperinsulinemic-euglycemic clamp to assess insulin action and glucose metabolism in individual organs, hyperglycemic clamp to assess pancreatic ?-cell functions in conscious mice, metabolic cages to assess energy expenditure, activity and food intake, 1H-MRS to measure body composition, exercise studies using treadmill, and blinded drug trial studies. The Analytical and Functional Core consists of the complementary 3 sub-Cores. Analytical sub-Core uses Luminex and Cobas Chemistry Analyzer to provide a high-throughput and multiplexed measurement of serum/tissue levels of hormones, cytokines and metabolites, and performs molecular experiments to examine insulin/metabolic signaling. The Islet sub-Core performs elegant islet isolation and ex vivo and in vitro analysis of islet function and histology, and molecular analysis. The Cardiovascular sub-Core uses state-of-the-art Vevo2100 In Vivo Imaging System to conduct echocardiography and tissue Doppler imaging for cardiac structure and function analyses, telemetry to examine blood pressure and heart rates, standardized techniques to assess vascular and endothelial functions, and various elegant surgical models of cardiovascular diseases. Mice provided by the users are overseen by the Animal Core that processes the receiving, quarantine, and housing of mice. The Humanized Mouse Cell Transplantation and Assessment sub-Core will service the MMPC community by offering humanized mice and elegant technique to assess in vivo function of transplanted human islets and stem cell-derived ?-cells in immunodeficient mice. Lastly, administrative, financial and scientific operation, research & development program, and website & database are managed by the Administrative Core. Overall, the overarching goal of the UMass MMPC is to provide comprehensive characterization of mice using state-of-the-art equipment and elegant and standardized techniques as an important scientific resource to support the mission of the National MMPC consortium program.

Public Health Relevance

Prevalence of diabetes is increasing at an alarming rate, and by year 2025, one out of 3 Americans is expected to be diabetic. Using established expertise, elegant experimental procedures, and state-of-the-art instruments, our goal is to understand how diabetes develops and to identify a cure to treat obesity and diabetes.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Resource-Related Research Multi-Component Projects and Centers Cooperative Agreements (U2C)
Project #
2U2CDK093000-06
Application #
9174710
Study Section
Special Emphasis Panel (ZDK1)
Program Officer
Abraham, Kristin M
Project Start
2011-09-16
Project End
2021-06-30
Budget Start
2016-08-01
Budget End
2017-06-30
Support Year
6
Fiscal Year
2016
Total Cost
Indirect Cost
Name
University of Massachusetts Medical School Worcester
Department
Other Basic Sciences
Type
Schools of Medicine
DUNS #
603847393
City
Worcester
State
MA
Country
United States
Zip Code
Shen, Yuefei; Cohen, Jessica L; Nicoloro, Sarah M et al. (2018) CRISPR-delivery particles targeting nuclear receptor-interacting protein 1 (Nrip1) in adipose cells to enhance energy expenditure. J Biol Chem 293:17291-17305
Yamamoto, Soh; Kuramoto, Kenta; Wang, Nan et al. (2018) Autophagy Differentially Regulates Insulin Production and Insulin Sensitivity. Cell Rep 23:3286-3299
Huynh, Frank K; Hu, Xiaoke; Lin, Zhihong et al. (2018) Loss of sirtuin 4 leads to elevated glucose- and leucine-stimulated insulin levels and accelerated age-induced insulin resistance in multiple murine genetic backgrounds. J Inherit Metab Dis 41:59-72
Kim, Jong Hun; Lee, Eunjung; Friedline, Randall H et al. (2018) Endoplasmic reticulum chaperone GRP78 regulates macrophage function and insulin resistance in diet-induced obesity. FASEB J 32:2292-2304
Hirako, Isabella Cristina; Assis, Patrícia Aparecida; Hojo-Souza, Natália Satchiko et al. (2018) Daily Rhythms of TNF? Expression and Food Intake Regulate Synchrony of Plasmodium Stages with the Host Circadian Cycle. Cell Host Microbe 23:796-808.e6
Caracciolo, Valentina; Young, Jeanette; Gonzales, Donna et al. (2018) Myeloid-specific deletion of Zfp36 protects against insulin resistance and fatty liver in diet-induced obese mice. Am J Physiol Endocrinol Metab 315:E676-E693
Huang, Li-Hao; Melton, Elaina M; Li, Haibo et al. (2018) Myeloid-specific Acat1 ablation attenuates inflammatory responses in macrophages, improves insulin sensitivity, and suppresses diet-induced obesity. Am J Physiol Endocrinol Metab 315:E340-E356
Owino, Sharon; Sánchez-Bretaño, Aida; Tchio, Cynthia et al. (2018) Nocturnal activation of melatonin receptor type 1 signaling modulates diurnal insulin sensitivity via regulation of PI3K activity. J Pineal Res 64:
Tse, Margaret Chui Ling; Herlea-Pana, Oana; Brobst, Daniel et al. (2017) Tumor Necrosis Factor-? Promotes Phosphoinositide 3-Kinase Enhancer A and AMP-Activated Protein Kinase Interaction to Suppress Lipid Oxidation in Skeletal Muscle. Diabetes 66:1858-1870
Dagdeviren, Sezin; Jung, Dae Young; Friedline, Randall H et al. (2017) IL-10 prevents aging-associated inflammation and insulin resistance in skeletal muscle. FASEB J 31:701-710

Showing the most recent 10 out of 37 publications