The Metabolism Core is designed to conduct in vivo and physiological experiments in conscious mice for the purpose of detailed metabolic phenotyping of transgenic mouse models useful in understanding obesity, diabetes and its complications. The Core operates under the supervision of Dr. Jason Kim (Director) and Drs. Roger Davis and Hye-Lim Noh as co-Directors who oversee day-to-day operation of the Core and interact with the users of the UMass MMPC. The principal functions of the Core are to perform the following experiments: 1) hyperinsulinemic-euglycemic clamp to assess organ-specific insulin action and glucose metabolism in conscious mice, 2) hyperglycemic clamp to assess in vivo pancreatic ?-cell function in conscious mice, 3) glucose/insulin tolerance tests, 4) survival surgery for in vivo experiments, 5) implementation of osmotic pumps for chronic delivery of drugs or other agents, 6) application of special diet (e.g., high-fat diet) to alter energy balance and induce obesity in mice, 7) acute lipid infusion, acute/chronic delivery of cytokines, and STZ injection to alter glucose homeostasis, 8) non-invasive measurement of energy expenditure, respiratory exchange ratio, physical activity, and food/water intake in conscious mice using metabolic cages, 9) non-invasive assessment of whole body, organ, and tissue biopsy composition of fat/lean/water mass in conscious mice using 1H-MRS, 10) implementation of treadmill and cage wheels for exercise study, 12) biochemical analysis for intracellular metabolic flux measurement (glycolysis, glycogen synthesis), and 13) blinded drug trial studies. The Metabolism Core regularly interacts with the Analytical and Functional Core for complementary, high-throughput analytical assays to measure hormones, cytokines and metabolites using serum samples obtained at the end of metabolic experiments. The goal of the Metabolism Core is to use an array of elegant, non-invasive and in vivo experiments to obtain a robust set of metabolic data from individual mouse in order to provide mechanistic insights into the role of selected gene on obesity, insulin resistance, and diabetes.

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University of Massachusetts Medical School Worcester
Anatomy/Cell Biology
Schools of Medicine
United States
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Owino, Sharon; Sánchez-Bretaño, Aida; Tchio, Cynthia et al. (2018) Nocturnal activation of melatonin receptor type 1 signaling modulates diurnal insulin sensitivity via regulation of PI3K activity. J Pineal Res 64:
Shen, Yuefei; Cohen, Jessica L; Nicoloro, Sarah M et al. (2018) CRISPR-delivery particles targeting nuclear receptor-interacting protein 1 (Nrip1) in adipose cells to enhance energy expenditure. J Biol Chem 293:17291-17305
Yamamoto, Soh; Kuramoto, Kenta; Wang, Nan et al. (2018) Autophagy Differentially Regulates Insulin Production and Insulin Sensitivity. Cell Rep 23:3286-3299
Huynh, Frank K; Hu, Xiaoke; Lin, Zhihong et al. (2018) Loss of sirtuin 4 leads to elevated glucose- and leucine-stimulated insulin levels and accelerated age-induced insulin resistance in multiple murine genetic backgrounds. J Inherit Metab Dis 41:59-72
Kim, Jong Hun; Lee, Eunjung; Friedline, Randall H et al. (2018) Endoplasmic reticulum chaperone GRP78 regulates macrophage function and insulin resistance in diet-induced obesity. FASEB J 32:2292-2304
Hirako, Isabella Cristina; Assis, Patrícia Aparecida; Hojo-Souza, Natália Satchiko et al. (2018) Daily Rhythms of TNF? Expression and Food Intake Regulate Synchrony of Plasmodium Stages with the Host Circadian Cycle. Cell Host Microbe 23:796-808.e6
Caracciolo, Valentina; Young, Jeanette; Gonzales, Donna et al. (2018) Myeloid-specific deletion of Zfp36 protects against insulin resistance and fatty liver in diet-induced obese mice. Am J Physiol Endocrinol Metab 315:E676-E693
Huang, Li-Hao; Melton, Elaina M; Li, Haibo et al. (2018) Myeloid-specific Acat1 ablation attenuates inflammatory responses in macrophages, improves insulin sensitivity, and suppresses diet-induced obesity. Am J Physiol Endocrinol Metab 315:E340-E356
Lee, Jieun; Choi, Joseph; Selen Alpergin, Ebru S et al. (2017) Loss of Hepatic Mitochondrial Long-Chain Fatty Acid Oxidation Confers Resistance to Diet-Induced Obesity and Glucose Intolerance. Cell Rep 20:655-667
Russo, Lucia; Muturi, Harrison T; Ghadieh, Hilda E et al. (2017) Liver-specific reconstitution of CEACAM1 reverses the metabolic abnormalities caused by its global deletion in male mice. Diabetologia 60:2463-2474

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