The Mutant Mouse Regional Resource Center (MMRRC) at the University of Missouri (MU) provides a unique repository service by importing, storing and distributing a vast number of mutant mouse strains, embryonic stem cells and related reagents as well as performing research to continuously improve the Resource Center. Genetically-engineered mice (GEM, i.e., transgenics, knock-outs and knock-ins) are invaluable to almost all fields of medicine. Tens of thousands of GEM have been created over the last two decades to facilitate better understanding of developmental biology and disease and to test and improve the function of drugs and therapeutics. Since the MMRRC Network began in 1999/2000, it has evolved into a major resource for the research community with capabilities and a combined inventory of mouse lines and embryonic stem (ES) cells that exceeds the capacity of other mouse repositories worldwide. The overall goal of this proposal is to continue the services and expand the functions and capabilities of the MU MMRRC.
The specific aims are to 1) provide biomedical investigators with the mouse models and related reagents they require for their research, 2) provide value-added services to aid the biomedical research community, and 3) perform research that has broad application to all models distributed. Over the next five years, the MU MMRRC will continue to rederive imported mice to a pathogen-free state; cryopreserve gametes and embryos; perform genetic and infectious disease monitoring to ensure quality; and distribute live mice, cryopreserved germplasm, or tissues. Research efforts will develop more economical and efficient means of cryopreservation/cryoresuscitation and will characterize intestinal microbiota to ensure reproducibility of results from studies that use distributed models. The MU MMRRC will also continue to transition to a self-sustaining unit by offering services to generate program income. Services include cryopreservation, cryoresuscitation from embryos or sperm, rederivation, genotyping and assay development, marker-assisted genetic analysis for development of speed congenics, karyotyping, colony management, phenotyping, and microbiome analysis.

Public Health Relevance

Genetically-engineered mice are instrumental to better understand human development and disease and to develop and improve drugs and therapeutics. The Mutant Mouse Regional Resource Center is a consortium of four centers that provides a unique repository for importing, storing and distributing a vast number of genetically-engineered mice, embryonic stem cells and related reagents. Each center of the MMRRC consortium also performs critical research and services to ensure quality and reproducibility of mouse models.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Materials Resource Cooperative Agreements (U42)
Project #
5U42OD010918-18
Application #
9204663
Study Section
Special Emphasis Panel (ZRG1-IFCN-B (55)R)
Program Officer
Mirochnitchenko, Oleg
Project Start
2000-05-01
Project End
2020-01-31
Budget Start
2017-02-01
Budget End
2018-01-31
Support Year
18
Fiscal Year
2017
Total Cost
$1,305,072
Indirect Cost
$405,563
Name
University of Missouri-Columbia
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
153890272
City
Columbia
State
MO
Country
United States
Zip Code
65211
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