The overall goal of this application is to continue operation of the National Swine Resource and Research Center (NSRRC). To date, the NSRRC is just beginning its ninth year of operation and it has successfully developed the infrastructure needed to assist swine-based research across multiple disciplines. Functionally, the NSRRC has been actively engaged in recruiting and importing swine models into the Center and in distributing cells, tissues, organs and pigs to investigators throughout the United States and Europe. The NSRRC also provides invaluable services to the research community by creating new genetically-engineered swine models needed by investigators and by training and educating scientists regarding the use of swine in biomedical research. Importantly, we have built an entirely new National Swine Resource and Research Center building, with state-of-the-art high biosecurity, to house the functions of the NSRRC.
The Specific Aims for the upcoming grant period are as follows: 1. To operate the National Swine Research and Resource Center. Functions of the NSRRC will continue to include: a) importation of existing swine models of human health and disease, b) rederivation of pigs to eliminate specific pathogens, and health monitoring to assure maintenance of a specific pathogen-free status, c) cryopreservation and storage of gametes, embryos and somatic cells to prevent future loss of valuable models, d) distribution of reagents, cells, tissues, organs and pathogen-free pigs, and e) creation of new genetically-engineered pigs needed by the biomedical research community. 2. To perform innovative research that will benefit the NSRRC and the biomedical research community. Applied research projects are aimed at improving: a) the cryopreservation of pig reproductive cells and tissues, b) developing state-of-the-art testing methods for swine health surveillance, and c) the development of improved methods for the production of genetically-engineered pigs. The NSRRC will also serve as a site for training and educational activities related to research employing swine models.

Public Health Relevance

The National Swine Resource and Research Center will continue to curate and provide much needed swine genetics that will be used to test treatments and therapies for human diseases. The National Swine Resource and Research Center will also create and distribute genetically engineered swine that manifest human diseases or can be used to treat human diseases.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Materials Resource Cooperative Agreements (U42)
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Study Section
Special Emphasis Panel (ZOD1)
Program Officer
Contreras, Miguel A
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University of Missouri-Columbia
Veterinary Sciences
Earth Sciences/Resources
United States
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Redel, Bethany K; Beaton, Benjamin P; Spate, Lee D et al. (2018) Single step production of Cas9 mRNA for zygote injection. Biotechniques 64:118-124
Ryu, Junghyun; Prather, Randall S; Lee, Kiho (2018) Use of gene-editing technology to introduce targeted modifications in pigs. J Anim Sci Biotechnol 9:5
Whyte, Jeffrey J; Meyer, Ashley E; Spate, Lee D et al. (2018) Inactivation of porcine interleukin-1? results in failure of rapid conceptus elongation. Proc Natl Acad Sci U S A 115:307-312
Yang, Jing; Menges, Steven; Gu, Ping et al. (2017) Porcine Neural Progenitor Cells Derived from Tissue at Different Gestational Ages Can Be Distinguished by Global Transcriptome. Cell Transplant 26:1582-1595
Yuan, Ye; Spate, Lee D; Redel, Bethany K et al. (2017) Quadrupling efficiency in production of genetically modified pigs through improved oocyte maturation. Proc Natl Acad Sci U S A 114:E5796-E5804
Wells, Kevin D; Prather, Randall S (2017) Genome-editing technologies to improve research, reproduction, and production in pigs. Mol Reprod Dev 84:1012-1017
Scott, Patrick A; de Castro, Juan P Fernandez; DeMarco, Paul J et al. (2017) Progression of Pro23His Retinopathy in a Miniature Swine Model of Retinitis Pigmentosa. Transl Vis Sci Technol 6:4
Whitworth, Kristin M; Benne, Joshua A; Spate, Lee D et al. (2017) Zygote injection of CRISPR/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or altering the sex ratio in pigs. Transgenic Res 26:97-107
Lee, Whayoung; Miyagawa, Yuko; Long, Cassandra et al. (2016) Expression of NeuGc on Pig Corneas and Its Potential Significance in Pig Corneal Xenotransplantation. Cornea 35:105-13
Redel, Bethany K; Prather, Randall S (2016) Meganucleases Revolutionize the Production of Genetically Engineered Pigs for the Study of Human Diseases. Toxicol Pathol 44:428-33

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