Project 3, Multi-omic analysis to expand phenotype in MMRRC mice Established by the NIH 20 years ago, the MMRRC serves the biomedical research community by preserving, protecting, and promoting the considerable national investment in creating and phenotyping mutant mouse models for the study of human biology and disease. With a census today exceeding 60,000 alleles, the MMRRC ensures that unique and scientifically valuable mouse strains produced and studied in independent PI laboratories can be deposited and archived for safekeeping so that they are available and accessible in perpetuity for scientists to obtain and use for their research studies. But the phenotypic description of mutant mice submitted to the MMRRC often narrowly reflects the specific scientific interests of the submitting investigator, reflecting the focus of the researcher who developed the line. Therefore, the phenotypic repertoire of many (if not most) MMRRC lines is far more diverse than what is known. For that reason, anything the MMRRC can do to expose these hidden phenotypes will at least add new knowledge about a line, some (if not most) of which will be scientifically valuable. Therefore, this project will pilot the feasibility of conducting relatively high-throughput, low cost, and unbiased phenotyping analyses to each mouse line submitted to the MMRRC. To that end, we propose to use extant, in-house transcriptomic, proteomic, and metabolomic platforms to establish a reliable and cost-effective protocol for high-fidelity multi-omic analysis of mouse tissues from MMRRC mice. We will conduct this study in two aims over the course of 5 years.
The first aim will establish and test procedures in wildtype mice, and in the second aim apply the protocol to reveal new phenotypes in MMRRC strains. We expect that comprehensive multi-omic characterization of submitted mouse lines will not only contribute to validating what is already known (i.e., reproducibility and reliability) but also will add an abundance of new phenotypic knowledge about MMRRC mouse lines. With this additional molecular information, the research utility of mutant mouse lines held by the MMRRC Consortium will increase, resulting in a growth in requests and greater use by the research community, and enhancing the distribution activity of the resource.

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University of California Davis
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Shin, Sora; Pribiag, Horia; Lilascharoen, Varoth et al. (2018) Drd3 Signaling in the Lateral Septum Mediates Early Life Stress-Induced Social Dysfunction. Neuron 97:195-208.e6
Valero-Jiménez, Ana; Zúñiga, Joaquín; Cisneros, José et al. (2018) Transmembrane protease, serine 4 (TMPRSS4) is upregulated in IPF lungs and increases the fibrotic response in bleomycin-induced lung injury. PLoS One 13:e0192963
Jeong, Yeongseok; Huh, Namjung; Lee, Joonyeup et al. (2018) Role of the hippocampal CA1 region in incremental value learning. Sci Rep 8:9870
Hoerder-Suabedissen, Anna; Hayashi, Shuichi; Upton, Louise et al. (2018) Subset of Cortical Layer 6b Neurons Selectively Innervates Higher Order Thalamic Nuclei in Mice. Cereb Cortex 28:1882-1897
Lanjakornsiripan, Darin; Pior, Baek-Jun; Kawaguchi, Daichi et al. (2018) Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers. Nat Commun 9:1623
Tröster, Philip; Haseleu, Julia; Petersen, Jonas et al. (2018) The Absence of Sensory Axon Bifurcation Affects Nociception and Termination Fields of Afferents in the Spinal Cord. Front Mol Neurosci 11:19
Gelegen, Cigdem; Miracca, Giulia; Ran, Mingzi Z et al. (2018) Excitatory Pathways from the Lateral Habenula Enable Propofol-Induced Sedation. Curr Biol 28:580-587.e5
Ralvenius, William T; Neumann, Elena; Pagani, Martina et al. (2018) Itch suppression in mice and dogs by modulation of spinal ?2 and ?3GABAA receptors. Nat Commun 9:3230
McCullough, Kenneth M; Daskalakis, Nikolaos P; Gafford, Georgette et al. (2018) Cell-type-specific interrogation of CeA Drd2 neurons to identify targets for pharmacological modulation of fear extinction. Transl Psychiatry 8:164
Chen, Li; Chen, Ruju; Kemper, Sherri et al. (2018) Therapeutic effects of serum extracellular vesicles in liver fibrosis. J Extracell Vesicles 7:1461505

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