This proposal seeks to competitively renew funding for infrastructure, personnel, and resources in support of the MMRRC at the University of California (UC) Davis (UCD-MMRRC). The UCD-MMRRC, alongside 2 centers at the University of Missouri-Columbia and University of North Carolina-Chapel Hill, and an Informatics, Coordination, and Service Center (ICSC) at The Jackson Laboratory, serves as the distribution archives and data center for the MMRRC National Program. Since its inception 10 years ago, the UCD- MMRRC has been one of the primary mutant mouse archive and distribution repositories in the world. We are the largest archive of three centers, with an inventory of over 28,500 mutant mouse lines representing ~98% of all live colonies, germplasm, and/or embryonic stem (ES) cells in the system. The UCD-MMRRC has fulfilled nearly 6000 orders from more than 2000 investigators at 500 institutions in 30 countries. We have also been a leader in innovation and resource development for the system, introducing new products (e.g., gene trap ES cells) and initiating new services (e.g., blastocyst injection, ICSI) that have added scientific value to mouse models and enhanced utilization of the MMRRC by researchers. UCD-MMRRC has leveraged its infrastructure and expertise to extend its service role by contracting with NIH institutes, biotechnology companies, and several organizations to archive, distribute, custom breed, genotype, and phenotype 1000's of additional ES cell mutants, BAC-transgenic, CRE, ENU-induced, and other mutant mouse collections. In addition, we have worked with the Centers and NIH program staff and developed and implemented advertising and marketing strategies, database management and informatics applications, website and online resources, customer and user services, operating procedures and policies, and shared governance of the national program.

Public Health Relevance

(provided by applicant): The UCD-MMRRC serves a primary role in ensuring that valuable mouse models of human disease, development, and behavioral abnormalities created in research laboratories are shared broadly among the biomedical scientific community. As a regionally-distributed repository and distribution archive, the UCD- MMRRC provides expertise, infrastructure, resources and services to preserve mouse strains in perpetuity, protect them from catastrophic loss, avoid genetic and phenotypic drift, and prevent pathogenic contamination and disease. By enabling availability and access of such valuable mouse models to the entire research community, the UCD-MMRRC fosters and promotes the discovery of new diagnostics, treatments, and prevention strategies against human diseases.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Materials Resource Cooperative Agreements (U42)
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Study Section
Special Emphasis Panel (ZRR1-CM-9 (01))
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Mirochnitchenko, Oleg
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University of California Davis
Internal Medicine/Medicine
Schools of Medicine
United States
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Li, Ming-Wen; Baridon, Brian; Trainor, Amanda et al. (2012) Mutant mice derived by ICSI of evaporatively dried spermatozoa exhibit expected phenotype. Reproduction 143:449-53
D'Amato, Nicholas C; Ostrander, Julie H; Bowie, Michelle L et al. (2012) Evidence for phenotypic plasticity in aggressive triple-negative breast cancer: human biology is recapitulated by a novel model system. PLoS One 7:e45684
Lee, Angus Yiu-Fai; Lloyd, K C Kent (2011) Rederivation of transgenic mice from iPS cells derived from frozen tissue. Transgenic Res 20:167-75
Cardiff, Robert D; Borowsky, Alexander D (2010) Precancer: sequentially acquired or predetermined? Toxicol Pathol 38:171-9
Cardiff, Robert Darrell (2010) The pathology of EMT in mouse mammary tumorigenesis. J Mammary Gland Biol Neoplasia 15:225-33
Li, Ming-Wen; Willis, Brandon J; Griffey, Stephen M et al. (2009) Assessment of three generations of mice derived by ICSI using freeze-dried sperm. Zygote 17:239-51
Dadi, Tedla D; Li, Ming W; Lloyd, K C Kent (2009) Decreased growth factor expression through RNA interference inhibits development of mouse preimplantation embryos. Comp Med 59:331-8
Elmoazzen, Heidi Y; Lee, Gloria Y; Li, Ming W et al. (2009) Further optimization of mouse spermatozoa evaporative drying techniques. Cryobiology 59:113-5
Radaelli, Enrico; Damonte, Patrizia; Cardiff, Robert D (2009) Epithelial-mesenchymal transition in mouse mammary tumorigenesis. Future Oncol 5:1113-27
Farrington-Rock, Claire; Kirilova, Veneta; Dillard-Telm, Lisa et al. (2008) Disruption of the Flnb gene in mice phenocopies the human disease spondylocarpotarsal synostosis syndrome. Hum Mol Genet 17:631-41

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