This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.REGARDING HIV this proposal is to establish and maintain a specific-pathogen-free (SPF) rhesus macaque supply and breeding colony program at the Sabana Seca Field Station (SSFS) of the Caribbean Prima Research Center (CPRC). This program will use genetically-characterized, MHC- typed, Indian-origin monkeys from the CPRC's free-ranging colony on the island of Cayo Santiago (CS). The CPRC will donate the founding stock for the colony from its Cayo Santiago and Cayo Santiago-derived populations. Previous surveys have shown that the CS macaques are free of several important viruses including retroviruses and simian virus 40 (SV-40), and that the majority of immature animals are negative for B-virus (Herpesvirus simiae Cercopithecine herpesvirus type 1). About 20- 25% of these monkeys are also Mamu-A*01 positive. Currently there is a shortage of SPF rhesus monkeys for biomedical research and the demand for these animals expected to increase dramatically in the future. The establishment and maintenance of this program at 551 will help meet the increased demand for both SPF (B-virus, SRV-D, SIV, STLV-1 and SV404ee) and Man' A*01 positive rhesus monkeys. Forty-five SPF females and nine SPF males (nine breeding groups) will added to the colony each year through internal recruitment from CS and SSFS. MHC-typing and selective breeding will be used to increase the production of SPF Mamu- A*01 positive offspring. By year five t program will have supplied 106 offspring including about 30 Mamu-A*01 positive animals. By year 6 or 7, the program should be self-supporting. The CPRC's program will make a significant contribution to advancing AIDS research by providing high quality and healthy SPF rhesus monkeys to NIH-sponsored research programs.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Puerto Rico Med Sciences
Other Domestic Higher Education
San Juan
United States
Zip Code
Rodriguez-Mercado, Rafael; Ford, Gregory D; Xu, Zhenfeng et al. (2012) Acute neuronal injury and blood genomic profiles in a nonhuman primate model for ischemic stroke. Comp Med 62:427-38
Sariol, Carlos A; Martínez, Melween I; Rivera, Francheska et al. (2011) Decreased dengue replication and an increased anti-viral humoral response with the use of combined Toll-like receptor 3 and 7/8 agonists in macaques. PLoS One 6:e19323
Sariol, Carlos A; Galib, Yussef; Pantoja, Petraleigh et al. (2009) Fatal granulomatous meningoencephalitis associated to mycobacterium mucogenicum-like microorganism: a case report. P R Health Sci J 28:276-80
Mejía, A F; Gierbolini, L; Jacob, B et al. (2009) Pediatric hepatic hemangiosarcoma in a rhesus macaque (Macaca mulatta). J Med Primatol 38:121-4
Satkoski, J; George, D; Smith, D G et al. (2008) Genetic characterization of wild and captive rhesus macaques in China. J Med Primatol 37:67-80
Sariol, Carlos A; Munoz-Jordan, Jorge L; Abel, Kristina et al. (2007) Transcriptional activation of interferon-stimulated genes but not of cytokine genes after primary infection of rhesus macaques with dengue virus type 1. Clin Vaccine Immunol 14:756-66
Sariol, C A; Gonzalez-Martinez, J; Arana, T et al. (2006) Differential distribution of antibodies to different viruses in young animals in the free-ranging rhesus macaques of Cayo Santiago. J Med Primatol 35:369-75
Kanthaswamy, Sreetharan; von Dollen, Andrea; Kurushima, Jennifer D et al. (2006) Microsatellite markers for standardized genetic management of captive colonies of rhesus macaques (Macaca mulatta). Am J Primatol 68:73-95
Sariol, Carlos A; Arana, Teresa; Maldonado, Elizabeth et al. (2005) Herpes B-virus seroreactivity in a colony of Macaca mulatta: data from the Sabana Seca Field Station, a new specific pathogen-free program. J Med Primatol 34:13-9