Abstract

The bacterial pathogen Francisella tularensis is a potential biological weapon by virtue of its ease of propagation, broad host range, myriad of transmission routes and extraordinary virulence. The highest morbidity and mortality is associated with pneumonic tularemia initiated by inhalation of F. tularensis. Little is known about genetic variations in host susceptibility to F. tularensis infections. C57/B6 and DBA inbredmouse strains exhibit varying susceptibility to F. tularensis infection. A series of mouse strains have been created by crossing the B/6 and DBA lines. We are determining the susceptibility of these cross-bred strains to F. tularensis using an intranasal inoculation route of infection with highly virulent Schu S4 F. tularensis strain. The results will be used to map genetic resistance and susceptibility alleles to F. tularensis infectionin order to define host traits that affect susceptibility to this infectious agent.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
3U54AI057157-06S1
Application #
7652174
Study Section
Special Emphasis Panel (ZAI1-NBS-M (M2))
Project Start
2008-03-15
Project End
2009-02-28
Budget Start
2008-03-15
Budget End
2009-02-28
Support Year
6
Fiscal Year
2008
Total Cost
$50,566
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

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Graham, Rachel L; Deming, Damon J; Deming, Meagan E et al. (2018) Evaluation of a recombination-resistant coronavirus as a broadly applicable, rapidly implementable vaccine platform. Commun Biol 1:179
Qi, Xiaoxuan; Wang, Wenjian; Dong, Haohao et al. (2018) Expression and X-Ray Structural Determination of the Nucleoprotein of Lassa Fever Virus. Methods Mol Biol 1604:179-188
Kocher, Jacob F; Lindesmith, Lisa C; Debbink, Kari et al. (2018) Bat Caliciviruses and Human Noroviruses Are Antigenically Similar and Have Overlapping Histo-Blood Group Antigen Binding Profiles. MBio 9:
Dhanwani, Rekha; Huang, Qinfeng; Lan, Shuiyun et al. (2018) Establishment of Bisegmented and Trisegmented Reverse Genetics Systems to Generate Recombinant Pichindé Viruses. Methods Mol Biol 1604:247-253
Shao, Junjie; Liu, Xiaoying; Liang, Yuying et al. (2018) Assays to Assess Arenaviral Glycoprotein Function. Methods Mol Biol 1604:169-178
Huang, Qinfeng; Shao, Junjie; Liang, Yuying et al. (2018) Assays to Demonstrate the Roles of Arenaviral Nucleoproteins (NPs) in Viral RNA Synthesis and in Suppressing Type I Interferon. Methods Mol Biol 1604:189-200
Gunn, Bronwyn M; Jones, Jennifer E; Shabman, Reed S et al. (2018) Ross River virus envelope glycans contribute to disease through activation of the host complement system. Virology 515:250-260
Shao, Junjie; Liang, Yuying; Ly, Hinh (2018) Roles of Arenavirus Z Protein in Mediating Virion Budding, Viral Transcription-Inhibition and Interferon-Beta Suppression. Methods Mol Biol 1604:217-227
Wirawan, Melissa; Fibriansah, Guntur; Marzinek, Jan K et al. (2018) Mechanism of Enhanced Immature Dengue Virus Attachment to Endosomal Membrane Induced by prM Antibody. Structure :

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