This core is to provide the researchers in the RMRCE with a novel way of testing the results and concepts derived from studies in rodents in human cells before attempting clinical studies in human volunteers or patients. It is our belief that human primary cells are significantly different from human tumor cell lines or primary rodent cells. We believe that these cells, which will be provided by the core, will be very useful to the RMRCE investigators to verify their findings in differentiated primary human lung cells. We isolate lung cells from de-identified human lung donors and will provide alveolar type II cells, alveolar type l-like cells, and alveolar macrophages to RMRCE investigators. Our laboratory has used these cells for infection with SARSCoV and influenza. We will isolate and characterize the cells and train investigators in culturing the epithelial cells. The cells can be frozen down and shipped to the investigators. We have successfully transported these cells to Fort Collins and shipped them to Hong Kong. The alveolar macrophages require no special handing and can be used easily by the investigators. The alveolar epithelial cells require special culture conditions, and investigators will have to spend some time in our lab to learn to prepare the matrices on which the cells are grown. Since one of the major objectives of the RMRCE is to make their discoveries applicable to human subjects and patients, they need access to human cells and tissue. This core will allow access to human lung cells and tissue. This core fits within the RMRCE Strategic Plan by interacting directly with projects in all three of the Integrated Research Focus groups: the Immunomodulation, Adjuvants and Vaccines IRF [RP 1.2 (Jutila), RP 1.3 (Pascual), RP 1.5 (Dow)];the Bacterial Therapeutics IRF [RP 2.7 (Lenz)];and the Viral Therapeutics IRF [RP 3.6 (Li)].

Public Health Relevance

Studies in rodents and rodent cells need to be confirmed in human cells before they can be transferred to the clinic. This core will provide access to human cells and tissues. In addition the investigators of this core have extensive experience in working with and characterizing alveolar epithelial cells and macrophages.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Specialized Center--Cooperative Agreements (U54)
Project #
Application #
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Colorado State University-Fort Collins
Fort Collins
United States
Zip Code
Webb, Jessica R; Price, Erin P; Somprasong, Nawarat et al. (2018) Development and validation of a triplex quantitative real-time PCR assay to detect efflux pump-mediated antibiotic resistance in Burkholderia pseudomallei. Future Microbiol 13:1403-1418
York, Joanne; Nunberg, Jack H (2018) A Cell-Cell Fusion Assay to Assess Arenavirus Envelope Glycoprotein Membrane-Fusion Activity. Methods Mol Biol 1604:157-167
Rhodes, Katherine A; Somprasong, Nawarat; Podnecky, Nicole L et al. (2018) Molecular determinants of Burkholderia pseudomallei BpeEF-OprC efflux pump expression. Microbiology 164:1156-1167
Cummings, Jason E; Slayden, Richard A (2017) Transient In Vivo Resistance Mechanisms of Burkholderia pseudomallei to Ceftazidime and Molecular Markers for Monitoring Treatment Response. PLoS Negl Trop Dis 11:e0005209
Pettey, W B P; Carter, M E; Toth, D J A et al. (2017) Constructing Ebola transmission chains from West Africa and estimating model parameters using internet sources. Epidemiol Infect 145:1993-2002
Furuta, Yousuke; Komeno, Takashi; Nakamura, Takaaki (2017) Favipiravir (T-705), a broad spectrum inhibitor of viral RNA polymerase. Proc Jpn Acad Ser B Phys Biol Sci 93:449-463
Skyberg, Jerod A; Lacey, Carolyn A (2017) Hematopoietic MyD88 and IL-18 are essential for IFN-?-dependent restriction of type A Francisella tularensis infection. J Leukoc Biol 102:1441-1450
Plumley, Brooke A; Martin, Kevin H; Borlee, Grace I et al. (2017) Thermoregulation of Biofilm Formation in Burkholderia pseudomallei Is Disrupted by Mutation of a Putative Diguanylate Cyclase. J Bacteriol 199:
Randall, Linnell B; Georgi, Enrico; Genzel, Gelimer H et al. (2017) Finafloxacin overcomes Burkholderia pseudomallei efflux-mediated fluoroquinolone resistance. J Antimicrob Chemother 72:1258-1260
Podnecky, Nicole L; Rhodes, Katherine A; Mima, Takehiko et al. (2017) Mechanisms of Resistance to Folate Pathway Inhibitors in Burkholderia pseudomallei: Deviation from the Norm. MBio 8:

Showing the most recent 10 out of 258 publications