Novel Targets in Endocrine Responsiveness. While Project 1 takes a focused analysis of specific signaling around cellular stress and cell fate decisions. Project 2 will take a functional genomics approach and study cell cycle progression in endocrine sensifive and resistant cells. Drs. Weiner and Golemis will lead the team in Project 2 and apply a powerful synthetic lethal screen approach that we have previously used to study EGFR-mediated signaling. Using a focused siRNA library targeting known ER-driven genes implicated in endocrine responsiveness, we will determine which genes, when their expression is inhibited, affect the ability of E2, TAM, and ICI to regulate cell cycle progression. Importanfiy. the siRNA library will be applied to the same cell models used in Proiect 1. The team will validate candidate genes that primarily affect cell proliferation {i.e., cell cycle), whereas candidate genes associated with specific cellular stress pathways and cell survival {e.g., apoptosis, autophagy, necrosis) will be studied in Project 1.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54CA149147-05
Application #
8627141
Study Section
Special Emphasis Panel (ZCA1-SRLB-C)
Project Start
Project End
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
5
Fiscal Year
2014
Total Cost
$184,276
Indirect Cost
Name
Georgetown University
Department
Type
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057
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