The overall goal of the Cell Separation and Sample Preparation (CSSP) Core is to develop technologies for the? collection and isolation of enriched blood leukocyte cell populations that are applicable to critically ill patient? populations for subsequent high throughput proteomic and genomic analyses, as well as for functional? proteomics. The Core also assists with the collection, processing and archival of solid tissue samples obtained? at surgical interventions in the trauma and burn patient populations. The Core has three primary functions? within the Program: (1) the development of new technologies for the isolation of leukocyte subpopulations from? critically-ill trauma and burn patients for subsequent genomic and proteomic analyses, initially employing? macroscale techniques, but rapidly moving to a microfluidics approach; (2) the implementation and support of? macroscale and microfluidics protocols at the clinical sites, including quality control and quality assurance; and,? (3) the long-term storage and archival of plasma, leukocyte and solid tissue samples (RNA, protein) for future? analyses. Because of these distinct functions, the Core has both development and service components. The? primary service site is at the University of Florida where the Sample Collection Kits are manufactured and? distributed, and where samples are archived and stored in 24-hour monitored freezers and facilities. The? primary development sites are at the Center for Engineering in Medicine where the microfluidics cassettes are? developed, and at the University of Rochester and the University of Florida College of Medicine where? macroscale sample processing is developed and validated.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Specialized Center--Cooperative Agreements (U54)
Project #
Application #
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Massachusetts General Hospital
United States
Zip Code
Gale, Stephen C; Kocik, Jurek F; Creath, Robert et al. (2016) A comparison of initial lactate and initial base deficit as predictors of mortality after severe blunt trauma. J Surg Res 205:446-455
Agarwal, Shailesh; Loder, Shawn; Brownley, Cameron et al. (2016) Inhibition of Hif1? prevents both trauma-induced and genetic heterotopic ossification. Proc Natl Acad Sci U S A 113:E338-47
Lopez, Maria-Cecilia; Efron, Philip A; Ozrazgat-Baslanti, Tezcan et al. (2016) Sex-based differences in the genomic response, innate immunity, organ dysfunction, and clinical outcomes after severe blunt traumatic injury and hemorrhagic shock. J Trauma Acute Care Surg 81:478-85
Sood, Ravi F; Gibran, Nicole S; Arnoldo, Brett D et al. (2016) Early leukocyte gene expression associated with age, burn size, and inhalation injury in severely burned adults. J Trauma Acute Care Surg 80:250-7
Mathias, Brittany; Lipori, Gigi; Moldawer, Lyle L et al. (2016) Integrating ""big data"" into surgical practice. Surgery 159:371-4
Mason, Stephanie A; Nathens, Avery B; Finnerty, Celeste C et al. (2016) Hold the Pendulum: Rates of Acute Kidney Injury are Increased in Patients Who Receive Resuscitation Volumes Less than Predicted by the Parkland Equation. Ann Surg 264:1142-1147
Tsurumi, Amy; Que, Yok-Ai; Yan, Shuangchun et al. (2015) Do standard burn mortality formulae work on a population of severely burned children and adults? Burns 41:935-45
Vanzant, Erin L; Ozrazgat-Baslanti, Tezcan; Liu, Huazhi et al. (2015) Clostridium difficile Infections after Blunt Trauma: A Different Patient Population? Surg Infect (Larchmt) 16:421-7
Vanzant, Erin L; Hilton, Rachael E; Lopez, Cecilia M et al. (2015) Advanced age is associated with worsened outcomes and a unique genomic response in severely injured patients with hemorrhagic shock. Crit Care 19:77
Hsu, Jessie J; Finkelstein, Dianne M; Schoenfeld, David A (2015) Outcome-Driven Cluster Analysis with Application to Microarray Data. PLoS One 10:e0141874

Showing the most recent 10 out of 199 publications