The primary goals for the Biosensor Initiative are to continue to develop biosensors for key regulatory and effector nodes in cell migration and to use existing and proposed biosensors to acquire quantitative data that can be used todevelop an experimentally verified theoretical model of cell migration by the Modeling Initiative and othersin the modeling community. The Initiative builds on the sensor technologies, fluorescence assays, highthroughput microscopy and collaborations from the first CMC funding period to move into Phase II,wherein we plan to visualize signaling events in living cells and to acquire and analyze large microscopicdata sets. The modeling will be carried out as a close collaboration with the Modeling Initiative. A majorfocus of the Initiative will be Rho family regulators since they control key migration nodes. In additionthe Initiative will focus on construction of biosensors and photoactivatable reagents, using both existingand new technologies, for key upstream and downstream members of these signaling pathways.In the end we produce a detailed experimental and mathematical analysis of a specific, critical signalingpathway in cell migration.
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