The overall goal of the Proteomics Initiative is to use mass spectrometry based proteomic? methods to expand the current migration knowledge database by examining phosphorylation site utilization of? proteins that regulate cell motility, by further developing methods for measuring differential phosphorylation of? those proteins, and by using quantitative methods to assess positional and kinetic variation in phosphorylation? site utilization in migration-related proteins. In addition, the Initiative will extend these proteomic methods to? provide positional cartography of the proteins present in protein complexes formed by known migration-related? proteins and protein products of novel migration genes identified in the unbiased screens of the Gene? Discovery Initiative. These goals are enabled by several pivotal developments in technology, analysis, and? methodology carried out during the first phase of funding. The Initiative focuses on two inter-related themes: 1.? Phosphoproteomics of migration related proteins and 2. Positional proteomics of migration-related protein? complexes and their phoshorylations.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Specialized Center--Cooperative Agreements (U54)
Project #
Application #
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Virginia
United States
Zip Code
Hu, Guiqing; Taylor, Dianne W; Liu, Jun et al. (2018) Identification of interfaces involved in weak interactions with application to F-actin-aldolase rafts. J Struct Biol 201:199-209
Kubow, Kristopher E; Shuklis, Victoria D; Sales, Dominic J et al. (2017) Contact guidance persists under myosin inhibition due to the local alignment of adhesions and individual protrusions. Sci Rep 7:14380
Gallegos, Lisa Leon; Ng, Mei Rosa; Sowa, Mathew E et al. (2016) A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for ?-catenin. Sci Rep 6:27114
Al-Dimassi, Saleh; Salloum, Gilbert; Saykali, Bechara et al. (2016) Targeting the MAP kinase pathway in astrocytoma cells using a recombinant anthrax lethal toxin as a way to inhibit cell motility and invasion. Int J Oncol 48:1913-20
Juanes-Garcia, Alba; Chapman, Jessica R; Aguilar-Cuenca, Rocio et al. (2015) A regulatory motif in nonmuscle myosin II-B regulates its role in migratory front-back polarity. J Cell Biol 209:23-32
Gao, Runchi; Zhao, Siwei; Jiang, Xupin et al. (2015) A large-scale screen reveals genes that mediate electrotaxis in Dictyostelium discoideum. Sci Signal 8:ra50
Dai, Aguang; Ye, Feng; Taylor, Dianne W et al. (2015) The Structure of a Full-length Membrane-embedded Integrin Bound to a Physiological Ligand. J Biol Chem 290:27168-75
Márquez, G; Pinto, A; Alamo, L et al. (2014) A method for 3D-reconstruction of a muscle thick filament using the tilt series images of a single filament electron tomogram. J Struct Biol 186:265-72
Zgheib, Perla; Daher, Costantine F; Mroueh, Mohamad et al. (2014) Daucus carota Pentane/Diethyl Ether Fraction Inhibits Motility and Reduces Invasion of Cancer Cells. Chemotherapy 60:302-9
Hinde, Elizabeth; Yokomori, Kyoko; Gaus, Katharina et al. (2014) Fluctuation-based imaging of nuclear Rac1 activation by protein oligomerisation. Sci Rep 4:4219

Showing the most recent 10 out of 368 publications