The overall goal of the Proteomics Initiative is to use mass spectrometry based proteomic methods to expand the current migration knowledge database by examining phosphorylation site utilization of proteins that regulate cell motility, by further developing methods for measuring differential phosphorylation of those proteins, and by using quantitative methods to assess positional and kinetic variation in phosphorylation site utilization in migration-related proteins. In addition, the Initiative will extend these proteomic methods to provide positional cartography of the proteins present in protein complexes formed by known migration-related proteins and protein products of novel migration genes identified in the unbiased screens of the Gene Discovery Initiative. These goals are enabled by several pivotal developments in technology, analysis, and methodology carried out during the first phase of funding. The Initiative focuses on two inter-related themes: 1. Phosphoproteomics of migration related proteins and 2. Positional proteomics of migration-related protein complexes and their phoshorylations.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54GM064346-10
Application #
8121481
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
2014-07-31
Budget Start
2010-08-01
Budget End
2013-07-31
Support Year
10
Fiscal Year
2010
Total Cost
$148,296
Indirect Cost
Name
University of Virginia
Department
Type
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
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