Abstract

2.1. PROJECT 2 (Wagner) ? New Labeling Schemes, NMR Technology, and MP Structure and Function The major objectives of this project are to develop MP reconstitution and isotope labeling methods and new NMR experiments for the proposed pipeline to facilitate MP structure determination by solution NMR. These new developments, in combination with methods to be established in other projects of the Center, will enable routine NMR structure determination of MPs and characterization of MP complexes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54GM094608-05
Application #
8688795
Study Section
Special Emphasis Panel (ZGM1-CBB-3)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
5
Fiscal Year
2014
Total Cost
$304,314
Indirect Cost
$86,408

  Institution

Name
Harvard Medical School
Department
Type
DUNS #
047006379
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Hagn, Franz; Nasr, Mahmoud L; Wagner, Gerhard (2018) Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR. Nat Protoc 13:79-98
Min, John; Shih, William M; Bellot, Gaëtan (2017) Designing DNA Nanotube Liquid Crystals as a Weak-Alignment Medium for NMR Structure Determination of Membrane Proteins. Methods Mol Biol 1500:203-215
Kim, Ji-Hun; Schlebach, Jonathan P; Lu, Zhenwei et al. (2016) A pH-Mediated Topological Switch within the N-Terminal Domain of Human Caveolin-3. Biophys J 110:2475-2485
Schlebach, Jonathan P; Barrett, Paul J; Day, Charles A et al. (2016) Topologically Diverse Human Membrane Proteins Partition to Liquid-Disordered Domains in Phase-Separated Lipid Vesicles. Biochemistry 55:985-8
Oxenoid, Kirill; Dong, Ying; Cao, Chan et al. (2016) Architecture of the mitochondrial calcium uniporter. Nature 533:269-73
Deatherage, Catherine L; Lu, Zhenwei; Kim, Ji-Hun et al. (2015) Notch Transmembrane Domain: Secondary Structure and Topology. Biochemistry 54:3565-8
Run, Changqing; Yang, Qin; Liu, Zhijun et al. (2015) Molecular Basis of MgATP Selectivity of the Mitochondrial SCaMC Carrier. Structure 23:1394-1403
Hagn, Franz; Wagner, Gerhard (2015) Structure refinement and membrane positioning of selectively labeled OmpX in phospholipid nanodiscs. J Biomol NMR 61:249-60
Schlebach, Jonathan P; Sanders, Charles R (2015) Influence of Pathogenic Mutations on the Energetics of Translocon-Mediated Bilayer Integration of Transmembrane Helices. J Membr Biol 248:371-81
Williamson, Jessica A; Cho, Seung-Hyun; Ye, Jiqing et al. (2015) Structure and multistate function of the transmembrane electron transporter CcdA. Nat Struct Mol Biol 22:809-14

Showing the most recent 10 out of 63 publications