Abstract

Core 5: Virology, Cell, Molecular and Chemical Biology Core Summary CRNA investigators have a wide range of expertise virology, cell and molecular biology, as well as chemical structural biology. Each of the biologically focused projects requires the application of techniques and expertise in these areas. To facilitate the identification of new targets for structural and biophysical analysis, and to facilitate the validation and biological interpretation of structural and biophysical data, the Virology, Cell, Molecular and Chemical Biology Core will undertake a number of activities including: (1) the discovery of new RNA targets for structure function studies using CLIP (2) the determination of RNA secondary structures in cells using DMS-MaPseq (3) siRNA and sCAS9/SAM screening to identify and validate new protein targets for structure function studies (4) the provision of virology and general molecular biology support to biologically and structurally focused projects and aims (5) virtual screening and chemical synthesis to enable the discovery of RNA- targeted inhibitors of the biological processes studied in the CRNA.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
2U54GM103297-06
Application #
9408805
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
2017-09-15
Budget End
2018-08-31
Support Year
6
Fiscal Year
2017
Total Cost
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Hron, Tomáš; Farkašová, Helena; Gifford, Robert J et al. (2018) Remnants of an Ancient Deltaretrovirus in the Genomes of Horseshoe Bats (Rhinolophidae). Viruses 10:
Gaines, Christy R; Tkacik, Emre; Rivera-Oven, Amalia et al. (2018) HIV-1 Matrix Protein Interactions with tRNA: Implications for Membrane Targeting. J Mol Biol 430:2113-2127
Krupovic, Mart; Blomberg, Jonas; Coffin, John M et al. (2018) Ortervirales: New Virus Order Unifying Five Families of Reverse-Transcribing Viruses. J Virol 92:
Blanco-Melo, Daniel; Gifford, Robert J; Bieniasz, Paul D (2018) Reconstruction of a replication-competent ancestral murine endogenous retrovirus-L. Retrovirology 15:34
LeBlanc, Regan M; Longhini, Andrew P; Tugarinov, Vitali et al. (2018) NMR probing of invisible excited states using selectively labeled RNAs. J Biomol NMR 71:165-172
Shi, Honglue; Clay, Mary C; Rangadurai, Atul et al. (2018) Atomic structures of excited state A-T Hoogsteen base pairs in duplex DNA by combining NMR relaxation dispersion, mutagenesis, and chemical shift calculations. J Biomol NMR 70:229-244
Kraus, Jodi; Gupta, Rupal; Yehl, Jenna et al. (2018) Chemical Shifts of the Carbohydrate Binding Domain of Galectin-3 from Magic Angle Spinning NMR and Hybrid Quantum Mechanics/Molecular Mechanics Calculations. J Phys Chem B 122:2931-2939
Marchant, Jan; Bax, Ad; Summers, Michael F (2018) Accurate Measurement of Residual Dipolar Couplings in Large RNAs by Variable Flip Angle NMR. J Am Chem Soc 140:6978-6983
Dick, Robert A; Zadrozny, Kaneil K; Xu, Chaoyi et al. (2018) Inositol phosphates are assembly co-factors for HIV-1. Nature 560:509-512
Patwardhan, Neeraj N; Cai, Zhengguo; Newson, Colby N et al. (2018) Fluorescent peptide displacement as a general assay for screening small molecule libraries against RNA. Org Biomol Chem :

Showing the most recent 10 out of 15 publications