Abstract

The overall goal of this project is to determine the physiological importance of the FSH-inhibin B feedback loop in sperm production in the non-human primate and to describe the cellular mechanisms involved in both the pituitary and testis.
Five specific aims are proposed. These include (1) to test the hypothesis that testicular inhibin B secretion is the key negative feedback signal that determines, via its effect on FSH release, the number of sperm produced; (2) to identify the paracrine mechanisms within the testis whereby FSH enlarges the population of differentiated spermatogonia; (3) to clone the gene encoding inhibin alpha and the inhibin receptor in the monkey and to produce sufficient monkey inhibin B for in vitro studies; (4) to localize the inhibin receptor in the pituitary and to study its role in mediating the negative feedback control of FSH by inhibin B; (5) to examine the hypothesis that the germinal epithelium is the principal regulator of Sertoli cell inhibin B secretion and that the physiological role of FSH in this regard is largely permissive.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54HD008610-28
Application #
6588480
Study Section
Special Emphasis Panel (ZHD1)
Project Start
2002-04-01
Project End
2003-03-31
Budget Start
Budget End
Support Year
28
Fiscal Year
2002
Total Cost
$176,413
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Type
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

Kawwass, Jennifer F; Sanders, Kristen M; Loucks, Tammy L et al. (2017) Increased cerebrospinal fluid levels of GABA, testosterone and estradiol in women with polycystic ovary syndrome. Hum Reprod 32:1450-1456
Vargas Trujillo, Marcela; Kalil, Bruna; Ramaswamy, Suresh et al. (2017) Estradiol Upregulates Kisspeptin Expression in the Preoptic Area of both the Male and Female Rhesus Monkey (Macaca mulatta): Implications for the Hypothalamic Control of Ovulation in Highly Evolved Primates. Neuroendocrinology 105:77-89
Kalil, Bruna; Ramaswamy, Suresh; Plant, Tony M (2016) The Distribution of Substance P and Kisspeptin in the Mediobasal Hypothalamus of the Male Rhesus Monkey and a Comparison of Intravenous Administration of These Peptides to Release GnRH as Reflected by LH Secretion. Neuroendocrinology 103:711-23
Walker, William H; Easton, Evan; Moreci, Rebecca S et al. (2015) Restoration of spermatogenesis and male fertility using an androgen receptor transgene. PLoS One 10:e0120783
Lomniczi, Alejandro; Wright, Hollis; Castellano, Juan Manuel et al. (2015) Epigenetic regulation of puberty via Zinc finger protein-mediated transcriptional repression. Nat Commun 6:10195
Shahab, M; Trujillo, M Vargas; Plant, T M (2015) A Reevaluation of the Question: Is the Pubertal Resurgence in Pulsatile GnRH Release in the Male Rhesus Monkey (Macaca mulatta) Associated With a Gonad-Independent Augmentation of GH Secretion? Endocrinology 156:3717-24
Verhagen, I; Ramaswamy, S; Teerds, K J et al. (2014) Time course and role of luteinizing hormone and follicle-stimulating hormone in the expansion of the Leydig cell population at the time of puberty in the rhesus monkey (Macaca mulatta). Andrology 2:924-30
Ramaswamy, Suresh; Dwarki, Karthik; Ali, Barkat et al. (2013) The decline in pulsatile GnRH release, as reflected by circulating LH concentrations, during the infant-juvenile transition in the agonadal male rhesus monkey (Macaca mulatta) is associated with a reduction in kisspeptin content of KNDy neurons of the arc Endocrinology 154:1845-53
Terasawa, Ei; Guerriero, Kathryn A; Plant, Tony M (2013) Kisspeptin and puberty in mammals. Adv Exp Med Biol 784:253-73
Stephens, Sahar M; Pau, Francis K Y; Yalcinkaya, Tamer M et al. (2013) Assessing the pulsatility of luteinizing hormone in female vervet monkeys (Chlorocebus aethiops sabaeus). Comp Med 63:432-8

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