Abstract

The objective of the Core is to scale-up and purify sufficient quantities of recombinant gamete antigens under good laboratory practice (GLP) guidelines for use as vaccinogens in immunogenicity and fertility trials in animal models and as assay target for monitoring immune responses.
The aims /services provided are: 1. To clone the cDNAs for gamete antigens into appropriate plasmid vectors for expression in bacterial or mammalian hosts. 2. To grown the bacterial/mammalian cells expressing the recombinant gamete antigen in large quantities using 20 L or 80 L bioreactors under GLP guidelines. 3. To purify recombinant gamete antigens and perform assays to verify their retention of immunogenic epitopes, i.e., to determine if the recombinant protein has acquired appropriate folding and/or post- translational modifications. 4. To produce uniform vaccine doses by loading, filling and quantifying vaccine lots under standard operating procedures, including shipping and inventory.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54HD029099-10
Application #
6467409
Study Section
Project Start
2001-03-01
Project End
2003-02-28
Budget Start
Budget End
Support Year
10
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
University of Virginia
Department
Type
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904

  Publications

Pires, Eusebio S; Hlavin, Callie; Macnamara, Ellen et al. (2013) SAS1B protein [ovastacin] shows temporal and spatial restriction to oocytes in several eutherian orders and initiates translation at the primary to secondary follicle transition. Dev Dyn 242:1405-26
Shumilin, Igor A; Cymborowski, Marcin; Chertihin, Olga et al. (2012) Identification of unknown protein function using metabolite cocktail screening. Structure 20:1715-25
Naaby-Hansen, Soren; Herr, John C (2010) Heat shock proteins on the human sperm surface. J Reprod Immunol 84:32-40
Naaby-Hansen, Soren; Diekman, Alan; Shetty, Jagathpala et al. (2010) Identification of calcium-binding proteins associated with the human sperm plasma membrane. Reprod Biol Endocrinol 8:6
Wolkowicz, M J; Digilio, L; Klotz, K et al. (2008) Equatorial segment protein (ESP) is a human alloantigen involved in sperm-egg binding and fusion. J Androl 29:272-82
Klotz, Kenneth L; Coppola, Michael A; Labrecque, Michel et al. (2008) Clinical and consumer trial performance of a sensitive immunodiagnostic home test that qualitatively detects low concentrations of sperm following vasectomy. J Urol 180:2569-76
Kurth, Barbara E; Digilio, Laura; Snow, Phillip et al. (2008) Immunogenicity of a multi-component recombinant human acrosomal protein vaccine in female Macaca fascicularis. J Reprod Immunol 77:126-41
Herr, John C; Chertihin, Olga; Digilio, Laura et al. (2008) Distribution of RNA binding protein MOEP19 in the oocyte cortex and early embryo indicates pre-patterning related to blastomere polarity and trophectoderm specification. Dev Biol 314:300-16
Shetty, Jagathpala; Bronson, Richard A; Herr, John C (2008) Human sperm protein encyclopedia and alloantigen index: mining novel allo-antigens using sera from ASA-positive infertile patients and vasectomized men. J Reprod Immunol 77:23-31
Xu, Bingfang; Hao, Zhonglin; Jha, Kula N et al. (2008) TSKS concentrates in spermatid centrioles during flagellogenesis. Dev Biol 319:201-10

Showing the most recent 10 out of 121 publications