Milestones: The pilot phase of the MLPCN was marked by several essential milestones. 1) In year 1. we transitioned ahead of schedule from 96 well to 384 well format. 2) We overcame a barrier in most available flow cytometers to 384 well data collection, the temporal resolution of data acquisition by integrating capabilities from the LANL NFCR P41 center. 3). We accomplished several multiplexed HT targets screens, to our knowledge, the first ever performed. 4) In year 2, we designed, with Beckman-Coulter, and took delivery of a liquid handling deck that integrated sample preparation and automated access to the MLSMR. At the time of this submission, a 200,000 compound screen has been accomplished for the low molecular GTPase sixplex and we are well on our way to a 250,000 compound screen for the Bcl-2 family sixplex. At the time of this submission, we have uploaded nearly 2 million HTS data points to PubChem.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54MH084690-03
Application #
8116590
Study Section
Special Emphasis Panel (ZRG1)
Project Start
2010-06-01
Project End
2014-05-31
Budget Start
2010-06-01
Budget End
2011-05-31
Support Year
3
Fiscal Year
2010
Total Cost
$739,019
Indirect Cost
Name
University of New Mexico Health Sciences Center
Department
Type
DUNS #
829868723
City
Albuquerque
State
NM
Country
United States
Zip Code
87131
Palsuledesai, Charuta C; Surviladze, Zurab; Waller, Anna et al. (2018) Activation of Rho Family GTPases by Small Molecules. ACS Chem Biol 13:1514-1524
Bredemeyer, Andrea L; Edwards, Bruce S; Haynes, Mark K et al. (2018) High-Throughput Screening Approach for Identifying Compounds That Inhibit Nonhomologous End Joining. SLAS Discov 23:624-633
Wu, Yang; Stauffer, Shaun R; Stanfield, Robyn L et al. (2016) Discovery of Small-Molecule Nonfluorescent Inhibitors of Fluorogen-Fluorogen Activating Protein Binding Pair. J Biomol Screen 21:74-87
Yang, Jeremy J; Ursu, Oleg; Lipinski, Christopher A et al. (2016) Badapple: promiscuity patterns from noisy evidence. J Cheminform 8:29
Zahoránszky-K?halmi, Gergely; Bologa, Cristian G; Oprea, Tudor I (2016) Impact of similarity threshold on the topology of molecular similarity networks and clustering outcomes. J Cheminform 8:16
Hong, Lin; Chavez, Stephanie; Smagley, Yelena et al. (2016) Relationship of light scatter change and Cdc42-regulated actin status. Cytometry B Clin Cytom 90:499-505
Sykes, David B; Kfoury, Youmna S; Mercier, François E et al. (2016) Inhibition of Dihydroorotate Dehydrogenase Overcomes Differentiation Blockade in Acute Myeloid Leukemia. Cell 167:171-186.e15
Holmes, Ann R; Cardno, Tony S; Strouse, J Jacob et al. (2016) Targeting efflux pumps to overcome antifungal drug resistance. Future Med Chem 8:1485-501
Schreiber, Stuart L; Kotz, Joanne D; Li, Min et al. (2015) Advancing Biological Understanding and Therapeutics Discovery with Small-Molecule Probes. Cell 161:1252-65
Agola, Jacob O; Sivalingam, Daniel; Cimino, Daniel F et al. (2015) Quantitative bead-based flow cytometry for assaying Rab7 GTPase interaction with the Rab-interacting lysosomal protein (RILP) effector protein. Methods Mol Biol 1298:331-54

Showing the most recent 10 out of 113 publications