Abstract

This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Technical Research Project 2, Protease Activity Imaging Technology (PAIT), will developtechnology for imaging of cellular proteolysis in three and four dimensions. Two independent andcomplementary approaches will be taken. First, a high throughput substrate phage display system willbe devised to identify highly selective indicator substrates for the vast majority of proteases encodedin the genome. Second, a chemical strategy will be applied to develop activity-based probes with anenzymatically activated fluorescent reporter. Both sets of beacons will be tested in biochemical andcellular models and will ultimately be used to image proteolysis at the cellular level

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Specialized Center--Cooperative Agreements (U54)
Project #
5U54RR020843-05
Application #
7725955
Study Section
Special Emphasis Panel (ZRG1-BST-D (55))
Project Start
2008-08-01
Project End
2009-07-31
Budget Start
2008-08-01
Budget End
2009-07-31
Support Year
5
Fiscal Year
2008
Total Cost
$352,373
Indirect Cost

  Institution

Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Savinov, Alexei Y; Strongin, Alex Y (2013) Targeting the T-cell membrane type-1 matrix metalloproteinase-CD44 axis in a transferred type 1 diabetes model in NOD mice. Exp Ther Med 5:438-442
Moin, Kamiar; Sameni, Mansoureh; Victor, Bernadette C et al. (2012) 3D/4D functional imaging of tumor-associated proteolysis: impact of microenvironment. Methods Enzymol 506:175-94
Mueller, Kelly L; Madden, Julie M; Zoratti, Gina L et al. (2012) Fibroblast-secreted hepatocyte growth factor mediates epidermal growth factor receptor tyrosine kinase inhibitor resistance in triple-negative breast cancers through paracrine activation of Met. Breast Cancer Res 14:R104
Giordano, Courtney R; Mueller, Kelly L; Terlecky, Laura J et al. (2012) A targeted enzyme approach to sensitization of tyrosine kinase inhibitor-resistant breast cancer cells. Exp Cell Res 318:2014-21
Bush, Jason A; Kitaura, Hideki; Ma, Yuliang et al. (2012) Comparative proteomic analysis of a cytosolic fraction from ?3 integrin-deficient cells. Cancer Genomics Proteomics 9:1-13
Mullins, Stefanie R; Sameni, Mansoureth; Blum, Galia et al. (2012) Three-dimensional cultures modeling premalignant progression of human breast epithelial cells: role of cysteine cathepsins. Biol Chem 393:1405-16
Békés, Miklós; Prudden, John; Srikumar, Tharan et al. (2011) The dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases. J Biol Chem 286:10238-47
Ren, Gang; Blum, Galia; Verdoes, Martijn et al. (2011) Non-invasive imaging of cysteine cathepsin activity in solid tumors using a 64Cu-labeled activity-based probe. PLoS One 6:e28029
Irwin, Mary E; Bohin, Natacha; Boerner, Julie L (2011) Src family kinases mediate epidermal growth factor receptor signaling from lipid rafts in breast cancer cells. Cancer Biol Ther 12:718-26
Zhu, Wenhong; Fang, Changming; Gramatikoff, Kosi et al. (2011) Proteins and an inflammatory network expressed in colon tumors. J Proteome Res 10:2129-39

Showing the most recent 10 out of 140 publications