Abstract

The purpose of this project is to study the mechanisms involved in regulating the binding of oxygen to hemoglobin and the transport of oxygen to the tissues. The project also focuses on ways in which these functions are impaired and change with age. We have therefore studied the mechanisms involved in the oxidation of hemoglobin. Oxidation affects oxygen transport because it produces nonfunctional hemoglobin, which no longer binds oxygen. These studies have been extended to include an investigation of the stability of the entire erythrocyte and the erythrocyte membrane as well as other structure function relationships in membranes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Intramural Research (Z01)
Project #
1Z01AG000047-15
Application #
4687876
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
15
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Aging
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Nagababu, Enika; Rifkind, Joseph M (2007) Measurement of plasma nitrite by chemiluminescence without interference of S-, N-nitroso and nitrated species. Free Radic Biol Med 42:1146-54
Rifkind, Joseph M; Nagababu, Enika; Barbiro-Michaely, Efrat et al. (2007) Nitrite infusion increases cerebral blood flow and decreases mean arterial blood pressure in rats: a role for red cell NO. Nitric Oxide 16:448-56
Nagababu, Enika; Ramasamy, Somasundaram; Rifkind, Joseph M (2007) Intermediates detected by visible spectroscopy during the reaction of nitrite with deoxyhemoglobin: the effect of nitrite concentration and diphosphoglycerate. Biochemistry 46:11650-9
Nagababu, Enika; Ramasamy, Somasundaram; Rifkind, Joseph M (2006) S-nitrosohemoglobin: a mechanism for its formation in conjunction with nitrite reduction by deoxyhemoglobin. Nitric Oxide 15:20-9
Lee, Garrick D; Longo, Dan L; Wang, Yue et al. (2006) Transient improvement in cognitive function and synaptic plasticity in rats following cancer chemotherapy. Clin Cancer Res 12:198-205
Rifkind, Joseph M; Nagababu, Enika; Ramasamy, Somasundaram (2006) Nitric oxide redox reactions and red cell biology. Antioxid Redox Signal 8:1193-203
Ravi, Luke B; Poosala, Suresh; Ahn, Dongchoon et al. (2005) Red cell interactions with amyloid-beta(1-40) fibrils in a murine model. Neurobiol Dis 19:28-37
Venkatesh, Balan; Venkatesh, S; Jayadevan, S et al. (2005) Studies on heme release from normal and metal ion reconstituted hemoglobin mediated through ionic surfactant. Biopolymers 80:18-25
Martinez, Alfredo; Oh, Hae-Ryong; Unsworth, Edward J et al. (2004) Matrix metalloproteinase-2 cleavage of adrenomedullin produces a vasoconstrictor out of a vasodilator. Biochem J 383:413-8
Jia, Yiping; Ramasamy, Somasundaram; Wood, Francine et al. (2004) Cross-linking with O-raffinose lowers oxygen affinity and stabilizes haemoglobin in a non-cooperative T-state conformation. Biochem J 384:367-75

Showing the most recent 10 out of 29 publications