Identification of Receptors for Merozoite Invasion of Erythrocytes

Miller, L H.

Abstract

The merozoite interacts in a receptor specific manner with the erythrocyte surface and is the stage against which immunity may work to block invasion. Thus, merozoite surface components are of interest for their role in erythrocyte recognition and as antigens for induction of protective immunity. We are identifying P. knowlesi receptors for attachment to monkey and human erythrocytes and P. falciparum proteins that are involved in the junction between merozoites and erythrocytes. We are studying one antigen on the merozoite surface that undergoes antigenic variation to understand the molecular basis for this variation. We are attempting to adapt P. falciparum to subprimates as an animal model for vaccine trials.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000241-08
• Application #: 3818147
• Support Year: 8
• Fiscal Year: 1988

Institution

• Name: Niaid Extramural Activities
• Country: United States

Publications

Mayer, D C Ghislaine; Cofie, Joann; Jiang, Lubin et al. (2009) Glycophorin B is the erythrocyte receptor of Plasmodium falciparum erythrocyte-binding ligand, EBL-1. Proc Natl Acad Sci U S A 106:5348-52

Beaty, Barry J; Prager, Denis J; James, Anthony A et al. (2009) From tucson to genomics and transgenics: the vector biology network and the emergence of modern vector biology. PLoS Negl Trop Dis 3:e343

Miura, Kazutoyo; Orcutt, Andrew C; Muratova, Olga V et al. (2008) Development and characterization of a standardized ELISA including a reference serum on each plate to detect antibodies induced by experimental malaria vaccines. Vaccine 26:193-200

Dicko, Alassane; Sagara, Issaka; Ellis, Ruth D et al. (2008) Phase 1 study of a combination AMA1 blood stage malaria vaccine in Malian children. PLoS ONE 3:e1563

Huaman, Maria Cecilia; Martin, Laura B; Malkin, Elissa et al. (2008) Ex vivo cytokine and memory T cell responses to the 42-kDa fragment of Plasmodium falciparum merozoite surface protein-1 in vaccinated volunteers. J Immunol 180:1451-61

Wu, Yimin; Ellis, Ruth D; Shaffer, Donna et al. (2008) Phase 1 trial of malaria transmission blocking vaccine candidates Pfs25 and Pvs25 formulated with montanide ISA 51. PLoS ONE 3:e2636

Mullen, Gregory E D; Ellis, Ruth D; Miura, Kazutoyo et al. (2008) Phase 1 trial of AMA1-C1/Alhydrogel plus CPG 7909: an asexual blood-stage vaccine for Plasmodium falciparum malaria. PLoS ONE 3:e2940

Qian, Feng; Rausch, Kelly M; Muratova, Olga et al. (2008) Addition of CpG ODN to recombinant Pseudomonas aeruginosa ExoProtein A conjugates of AMA1 and Pfs25 greatly increases the number of responders. Vaccine 26:2521-7

Gaur, Deepak; Singh, Sanjay; Singh, Subhash et al. (2007) Recombinant Plasmodium falciparum reticulocyte homology protein 4 binds to erythrocytes and blocks invasion. Proc Natl Acad Sci U S A 104:17789-94

Singh, Subhash; Plassmeyer, Matthew; Gaur, Deepak et al. (2007) Mononeme: a new secretory organelle in Plasmodium falciparum merozoites identified by localization of rhomboid-1 protease. Proc Natl Acad Sci U S A 104:20043-8