Isolation and Serotypic Characterization of Human and Animal Rotaviruses

Hoshino, Y.

Abstract

Bovine rotavirus strain B223 was shown, by a two-way cross-neutralization assay, to be distinct from any of the 9 established serotypes. In addition, approximately 75% of the VP7 gene has been sequenced and available data are consistent with its designation as a new serotype.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000339-09
• Application #: 3809621
• Support Year: 9
• Fiscal Year: 1990

Institution

• Name: National Institute of Allergy and Infectious Diseases
• Country: United States

Publications

Ross, Jerri; Ostlund, Eileen N; Cao, Dianjun et al. (2008) Acrylamide concentration affects the relative position of VP7 gene of serotype G2 strains as determined by polyacrylamide gel electrophoresis. J Clin Virol 42:374-80

Cao, Dianjun; Santos, Norma; Jones, Ronald W et al. (2008) The VP7 genes of two G9 rotaviruses isolated in 1980 from diarrheal stool samples collected in Washington, DC, are unique molecularly and serotypically. J Virol 82:4175-9

Santos, Norma; Honma, Shinjiro; Timenetsky, Maria do Carmo S T et al. (2008) Development of a microtiter plate hybridization-based PCR-enzyme-linked immunosorbent assay for identification of clinically relevant human group A rotavirus G and P genotypes. J Clin Microbiol 46:462-9

Honma, Shinjiro; Chizhikov, Vladimir; Santos, Norma et al. (2007) Development and validation of DNA microarray for genotyping group A rotavirus VP4 (P[4], P[6], P[8], P[9], and P[14]) and VP7 (G1 to G6, G8 to G10, and G12) genes. J Clin Microbiol 45:2641-8

Volotao, Eduardo M; Soares, Caroline C; Maranhao, Adriana G et al. (2006) Rotavirus surveillance in the city of Rio de Janeiro-Brazil during 2000-2004: detection of unusual strains with G8P[4] or G10P[9] specificities. J Med Virol 78:263-72

Hoshino, Yasutaka; Honma, Shinjiro; Jones, Ronald W et al. (2006) A rotavirus strain isolated from pig-tailed macaque (Macaca nemestrina) with diarrhea bears a P6[1]:G8 specificity. Virology 345:1-12

Pietruchinski, Eduardo; Benati, Fabricio; Lauretti, Flavio et al. (2006) Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: distribution of G/P types and finding of a rare G12 strain. J Med Virol 78:1241-9

Hoshino, Yasutaka; Jones, Ronald W; Ross, Jerri et al. (2005) Porcine rotavirus strain Gottfried-based human rotavirus candidate vaccines: construction and characterization. Vaccine 23:3791-9

Santos, Norma; Volotao, Eduardo M; Soares, Caroline C et al. (2005) Predominance of rotavirus genotype G9 during the 1999, 2000, and 2002 seasons among hospitalized children in the city of Salvador, Bahia, Brazil: implications for future vaccine strategies. J Clin Microbiol 43:4064-9

Kapikian, Albert Z; Simonsen, Lone; Vesikari, Timo et al. (2005) A hexavalent human rotavirus-bovine rotavirus (UK) reassortant vaccine designed for use in developing countries and delivered in a schedule with the potential to eliminate the risk of intussusception. J Infect Dis 192 Suppl 1:S22-9