Isolation and Serotypic Characterization of Human and Animal Rotaviruses

Hoshino, Y.

Abstract

In this project we determined major antigenic relationships of various rotavirus strains derived from humans and animals. The elucidation of the neutralization specificities of rotavirus is important in order to achieve a more comprehensive understanding of rotavirus epidemiology and for formulation of an effective strategy for vaccination. By using various single gene substitution human x human or human x bovine rotavirus reassortants and hyperimmune guinea pig antiserum raised against each reassortant, we successfully characterized the VP4 neutralization specificity of representative human rotavirus strains (Wa, DS-1, ST3, K8, and 69M) and established five distinct VP4(P) serotypes based on a >20-fold antibody difference criterion that is applied to classification of VP7 serotypes. Neutralization specificities on the VP4 capsid protein of two rabbit rotaviruses (C11 and Ala) originally isolated in the U.S. were found to be related antigenically in a two-way fashion to human rotavirus VP4 of VP7 serotype 6 PA169 strain (isolated in Italy) and of the VP7 serotype 8 HAL1160 strain (isolated in Finland).

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000339-15
• Application #: 2566747
• Study Section: Special Emphasis Panel (LID)
• Support Year: 15
• Fiscal Year: 1996

Institution

• Name: National Institute of Allergy and Infectious Diseases
• Country: United States

Publications

Santos, Norma; Honma, Shinjiro; Timenetsky, Maria do Carmo S T et al. (2008) Development of a microtiter plate hybridization-based PCR-enzyme-linked immunosorbent assay for identification of clinically relevant human group A rotavirus G and P genotypes. J Clin Microbiol 46:462-9

Ross, Jerri; Ostlund, Eileen N; Cao, Dianjun et al. (2008) Acrylamide concentration affects the relative position of VP7 gene of serotype G2 strains as determined by polyacrylamide gel electrophoresis. J Clin Virol 42:374-80

Cao, Dianjun; Santos, Norma; Jones, Ronald W et al. (2008) The VP7 genes of two G9 rotaviruses isolated in 1980 from diarrheal stool samples collected in Washington, DC, are unique molecularly and serotypically. J Virol 82:4175-9

Honma, Shinjiro; Chizhikov, Vladimir; Santos, Norma et al. (2007) Development and validation of DNA microarray for genotyping group A rotavirus VP4 (P[4], P[6], P[8], P[9], and P[14]) and VP7 (G1 to G6, G8 to G10, and G12) genes. J Clin Microbiol 45:2641-8

Volotao, Eduardo M; Soares, Caroline C; Maranhao, Adriana G et al. (2006) Rotavirus surveillance in the city of Rio de Janeiro-Brazil during 2000-2004: detection of unusual strains with G8P[4] or G10P[9] specificities. J Med Virol 78:263-72

Hoshino, Yasutaka; Honma, Shinjiro; Jones, Ronald W et al. (2006) A rotavirus strain isolated from pig-tailed macaque (Macaca nemestrina) with diarrhea bears a P6[1]:G8 specificity. Virology 345:1-12

Pietruchinski, Eduardo; Benati, Fabricio; Lauretti, Flavio et al. (2006) Rotavirus diarrhea in children and adults in a southern city of Brazil in 2003: distribution of G/P types and finding of a rare G12 strain. J Med Virol 78:1241-9

Hoshino, Yasutaka; Jones, Ronald W; Ross, Jerri et al. (2005) Porcine rotavirus strain Gottfried-based human rotavirus candidate vaccines: construction and characterization. Vaccine 23:3791-9

Santos, Norma; Volotao, Eduardo M; Soares, Caroline C et al. (2005) Predominance of rotavirus genotype G9 during the 1999, 2000, and 2002 seasons among hospitalized children in the city of Salvador, Bahia, Brazil: implications for future vaccine strategies. J Clin Microbiol 43:4064-9

Kapikian, Albert Z; Simonsen, Lone; Vesikari, Timo et al. (2005) A hexavalent human rotavirus-bovine rotavirus (UK) reassortant vaccine designed for use in developing countries and delivered in a schedule with the potential to eliminate the risk of intussusception. J Infect Dis 192 Suppl 1:S22-9