Abstract

A cyst-protein gene was identified and sequenced. The predicted protein is a 26 kDa leucine-rich protein with 5 degenerate repeats. Monoclonal antibodies were produced to other cyst and secretory proteins. The genomic organization of the H7 VSP of isolate GS/M was determined. In contrast to the tail-to-tail arrangement of two identical 1267 VSP genes, there was only one VSP H7 gene. Another closely related H7 gene was identified and sequenced. One specific area was responsible for most of the divergence between the genes. The upstream untranslated regions were practically identical. The control of VSP transcription must take account of the identical upstream regions which usually contain controlling transcriptional elements.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000350-12
Application #
3746520
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Worgall, Tilla S; Davis-Hayman, Sara R; Magana, Marissa M et al. (2004) Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoter: evidence for SREBP as an ancient transcription factor. J Lipid Res 45:981-8
Davis-Hayman, Sara R; Hayman, J Russell; Nash, Theodore E (2003) Encystation-specific regulation of the cyst wall protein 2 gene in Giardia lamblia by multiple cis-acting elements. Int J Parasitol 33:1005-12
Nash, Theodore E (2002) Surface antigenic variation in Giardia lamblia. Mol Microbiol 45:585-90
Davis-Hayman, Sara R; Nash, Theodore E (2002) Genetic manipulation of Giardia lamblia. Mol Biochem Parasitol 122:1-7
Hayman, J R; Hayes, S F; Amon, J et al. (2001) Developmental expression of two spore wall proteins during maturation of the microsporidian Encephalitozoon intestinalis. Infect Immun 69:7057-66
Nash, T E; Lujan, H T; Mowatt, M R et al. (2001) Variant-specific surface protein switching in Giardia lamblia. Infect Immun 69:1922-3
Elmendorf, H G; Singer, S M; Nash, T E (2001) The abundance of sterile transcripts in Giardia lamblia. Nucleic Acids Res 29:4674-83
Singer, S M; Elmendorf, H G; Conrad, J T et al. (2001) Biological selection of variant-specific surface proteins in Giardia lamblia. J Infect Dis 183:119-24
Elmendorf, H G; Singer, S M; Pierce, J et al. (2001) Initiator and upstream elements in the alpha2-tubulin promoter of Giardia lamblia. Mol Biochem Parasitol 113:157-69