Abstract

The structure and processing of the small 1A protein of human respiratory syncytial virus (RSV) was investigated. The 1A protein accumulated intracellulary as four species: two species (4.8K and 7.2K) were unglycosylated and differed in length because translational initiation for the synthesis of the 4.8K species occurred at the second methionine in the sequence. The two other forms (13-15K and 21-30K) contained an N-linked carbohydrate side chain. The 13-15K species appeared to be converted into the 21- 30K form by the post-translational additional of polyactosamine to the N-linked side chain. This complex processing pathway for 1A was conserved between the RSV antigenic subgroups. All forms of 1A were shown to be inserted into intracellular membranes regardless of glycosylation status, and all forms except 4.8K were transported to the cell surface. Protein-mapping studies showed that the C-terminus of the 1A protein is extracellular, whereas the N-terminus is cytoplasmic. Additional studies to investigate the structure and processing of other RSV proteins are discussed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000498-03
Application #
3818272
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Niaid Extramural Activities
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

McGivern, David R; Collins, Peter L; Fearns, Rachel (2005) Identification of internal sequences in the 3' leader region of human respiratory syncytial virus that enhance transcription and confer replication processivity. J Virol 79:2449-60
Zhang, Liqun; Bukreyev, Alexander; Thompson, Catherine I et al. (2005) Infection of ciliated cells by human parainfluenza virus type 3 in an in vitro model of human airway epithelium. J Virol 79:1113-24
Schomacker, Henrick; Collins, Peter L; Schmidt, Alexander C (2004) In silico identification of a putative new paramyxovirus related to the Henipavirus genus. Virology 330:178-85
Tran, Kim C; Collins, Peter L; Teng, Michael N (2004) Effects of altering the transcription termination signals of respiratory syncytial virus on viral gene expression and growth in vitro and in vivo. J Virol 78:692-9
Spann, Kirsten M; Collins, Peter L; Teng, Michael N (2003) Genetic recombination during coinfection of two mutants of human respiratory syncytial virus. J Virol 77:11201-11
Kotelkin, Alexander; Prikhod'ko, Elena A; Cohen, Jeffrey I et al. (2003) Respiratory syncytial virus infection sensitizes cells to apoptosis mediated by tumor necrosis factor-related apoptosis-inducing ligand. J Virol 77:9156-72
Zhang, Liqun; Peeples, Mark E; Boucher, Richard C et al. (2002) Respiratory syncytial virus infection of human airway epithelial cells is polarized, specific to ciliated cells, and without obvious cytopathology. J Virol 76:5654-66
Teng, Michael N; Collins, Peter L (2002) The central conserved cystine noose of the attachment G protein of human respiratory syncytial virus is not required for efficient viral infection in vitro or in vivo. J Virol 76:6164-71
Techaarpornkul, Sunee; Collins, Peter L; Peeples, Mark E (2002) Respiratory syncytial virus with the fusion protein as its only viral glycoprotein is less dependent on cellular glycosaminoglycans for attachment than complete virus. Virology 294:296-304
Gower, T L; Peeples, M E; Collins, P L et al. (2001) RhoA is activated during respiratory syncytial virus infection. Virology 283:188-96

Showing the most recent 10 out of 19 publications