Regulation of Cytokine Gene Expression in Mast Cells

Metcalfe, Dean D.

Abstract

Mast cells within tissue have constant relationships with specific structures such as nerves and blood vessels, although the stimuli that govern mast cell migration and homing are incompletely understood. We now report that the receptor CX3CR1 is expressed by cultured murine mast cells and by isolated human dermal mast cells. Fractalkine, a membrane-bound CX3C chemokine expressed by dermal epithelial and dendritric cells, attracts mast cells in vitro. Fractalkine did not lead to mast cell degranulation. It may thus be that fractalkine expression by specific cells within tissues may contribute to tissue-specific homing of mast cells

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000607-10
• Application #: 6431604
• Study Section: (LAD)
• Support Year: 10
• Fiscal Year: 2000

Institution

• Name: Niaid Extramural Activities
• Country: United States

Publications

Kulka, M; Metcalfe, D D (2006) TLR3 activation inhibits human mast cell attachment to fibronectin and vitronectin. Mol Immunol 43:1579-86

Metcalfe, Dean D; Boyce, Joshua A (2006) Mast cell biology in evolution. J Allergy Clin Immunol 117:1227-9

Kulka, Marianna; Fukuishi, Nobuyuki; Rottem, Menachem et al. (2006) Mast cells, which interact with Escherichia coli, up-regulate genes associated with innate immunity and become less responsive to Fc(epsilon)RI-mediated activation. J Leukoc Biol 79:339-50

Zudaire, Enrique; Martinez, Alfredo; Garayoa, Mercedes et al. (2006) Adrenomedullin is a cross-talk molecule that regulates tumor and mast cell function during human carcinogenesis. Am J Pathol 168:280-91

Davis, Beverley J; Flanagan, Brian F; Gilfillan, Alasdair M et al. (2004) Nitric oxide inhibits IgE-dependent cytokine production and Fos and Jun activation in mast cells. J Immunol 173:6914-20

Swindle, Emily J; Metcalfe, Dean D; Coleman, John W (2004) Rodent and human mast cells produce functionally significant intracellular reactive oxygen species but not nitric oxide. J Biol Chem 279:48751-9

Woolhiser, Michael R; Brockow, Knut; Metcalfe, Dean D (2004) Activation of human mast cells by aggregated IgG through FcgammaRI: additive effects of C3a. Clin Immunol 110:172-80

Kulka, Marianna; Alexopoulou, Lena; Flavell, Richard A et al. (2004) Activation of mast cells by double-stranded RNA: evidence for activation through Toll-like receptor 3. J Allergy Clin Immunol 114:174-82

Basta, Milan; Van Goor, Fredric; Luccioli, Stefano et al. (2003) F(ab)'2-mediated neutralization of C3a and C5a anaphylatoxins: a novel effector function of immunoglobulins. Nat Med 9:431-8

Okayama, Yoshimichi; Tkaczyk, Christine; Metcalfe, Dean D et al. (2003) Comparison of Fc epsilon RI- and Fc gamma RI-mediated degranulation and TNF-alpha synthesis in human mast cells: selective utilization of phosphatidylinositol-3-kinase for Fc gamma RI-induced degranulation. Eur J Immunol 33:1450-9