Abstract

Anopheles gambiae s.s. is the primary mosquito vector of malaria is Africa. As a prelude to the evaluation of strategies for malaria control based on the concept of replacement of vector populations with mosquitoes unable to transmit the malaria parasite, we are examining, in depth, the genetic and biologic structure of vector population in a series of distinct ecological zones in Mali (West Africa). To facilitate these studies, we are using the tools of molecular genetics directed at microsatellite polymorphisms, restriction length polymorphisms (RFLPs), and ribosomal and mitochondrial DNA. Field studies of vector populations using these techniques will be conducted at the Malaria Research and Training Center recently established in Bamako, Mali, in collaboration with staff of the National School of Medicine and Pharmacy of Mali. In addition, studies on larval ecology and nutrition of An. gambiae in Mali seek to identify microorganisms in the diet which might serve as carriers of genes coding for the production of biologically active insecticides for use in larval control programs.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Intramural Research (Z01)
Project #
1Z01AI000629-02
Application #
3768868
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
National Institute of Allergy and Infectious Diseases
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Manoukis, Nicholas C; Diabate, Abdoulaye; Abdoulaye, Adamou et al. (2009) Structure and dynamics of male swarms of Anopheles gambiae. J Med Entomol 46:227-35
Diabate, Abdoulaye; Dao, Adama; Yaro, Alpha S et al. (2009) Spatial swarm segregation and reproductive isolation between the molecular forms of Anopheles gambiae. Proc Biol Sci 276:4215-22
Lehmann, Tovi; Hume, Jen C C; Licht, Monica et al. (2009) Molecular evolution of immune genes in the malaria mosquito Anopheles gambiae. PLoS One 4:e4549
Sogoba, Nafomon; Doumbia, Seydou; Vounatsou, Penelope et al. (2007) Monitoring of larval habitats and mosquito densities in the Sudan savanna of Mali: implications for malaria vector control. Am J Trop Med Hyg 77:82-8
Diabate, Abdoulaye; Dabire, Roch K; Millogo, Niama et al. (2007) Evaluating the effect of postmating isolation between molecular forms of Anopheles gambiae (Diptera: Culicidae). J Med Entomol 44:60-4
Simard, Frederic; Licht, Monica; Besansky, Nora J et al. (2007) Polymorphism at the defensin gene in the Anopheles gambiae complex: testing different selection hypotheses. Infect Genet Evol 7:285-92
Yaro, A S; Dao, A; Adamou, A et al. (2006) Reproductive output of female Anopheles gambiae (Diptera: Culicidae): comparison of molecular forms. J Med Entomol 43:833-9
Marcet, P L; Lehmann, T; Groner, G et al. (2006) Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma infestans (Heteroptera: Reduviidae). Infect Genet Evol 6:32-7
Diabate, Abdoulaye; Dabire, Roch K; Kengne, Pierre et al. (2006) Mixed swarms of the molecular M and S forms of Anopheles gambiae (Diptera: Culicidae) in sympatric area from Burkina Faso. J Med Entomol 43:480-3
Lehmann, Tovi; Dalton, Ryan; Kim, Eun Hea et al. (2006) Genetic contribution to variation in larval development time, adult size, and longevity of starved adults of Anopheles gambiae. Infect Genet Evol 6:410-6

Showing the most recent 10 out of 16 publications