A RNA differential display analysis was performed using RNAs from the liver tumor cell lines SK-HEP-1, HEP-G2 and HEP-3B, as well as normal liver RNA. Promising bands were eluted from the gel and molecularly cloned. About 20 clones were confirmed to correspond to genes that are differentially expressed among the different RNA sources by RNA gel (Northern) blotting. Probing multiple tissue Northern (MTN) blots revealed that several of these genes have limited expression in normal tissues. They may be useful in the study of the biology of liver cancer or may be potential markers for this disease.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Intramural Research (Z01)
Project #
1Z01BC005739-04
Application #
2463681
Study Section
Special Emphasis Panel (LMO)
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1996
Total Cost
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
United States
Zip Code