Salivary Gland Secretory Mechanisms During Normal and Altered Functional States

Baum, B J.

Abstract

The health of the oral cavity is maintained by salivary secretions. The principal function of salivary glands is to produce these complex fluids. We utilize both in vitro and in vivo preparations of rat salivary glands primarily to understand mechanisms controlling saliva formation. We have focused our studies on autonomic neurotransmitter regulation of secretory events in normal, aging and two types of therapy-induced salivary gland dysfunctions (X-radiation, phenothiazines). During the present reporting period specific areas of study were (1) further examination of mechanistic processes in Ca2+ handling by parotid basolateral plasma membrane; (2) determination of the presence, and importance, of a Na+, K+, 2 Cl transport mechanism in parotid fluid secretion; (3) the possible regulatory role of VIP (vasoactive intestinal polypeptide) in parotid secretion; (4) the evauation of chlorpromazine as a competitive muscarinic antagonist; and (5) the development and characterization of an epithelial cell line from rat submandibular glands.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Dental & Craniofacial Research (NIDCR)
• Type: Intramural Research (Z01)
• Project #: 1Z01DE000336-04
• Application #: 4692638
• Support Year: 4
• Fiscal Year: 1985

Institution

• Name: Dental & Craniofacial Research
• Country: United States

Publications

Zheng, Changyu; Baum, Bruce J (2008) Evaluation of promoters for use in tissue-specific gene delivery. Methods Mol Biol 434:205-19

Adriaansen, Janik; Perez, Paola; Goldsmith, Corine et al. (2008) Differential sorting of human parathyroid hormone after transduction of mouse and rat salivary glands. Hum Gene Ther :

Cotrim, Ana P; Baum, Bruce J (2008) Gene therapy: some history, applications, problems, and prospects. Toxicol Pathol 36:97-103

Samuni, Yuval; Zheng, Changyu; Cawley, Niamh X et al. (2008) Sorting of growth hormone-erythropoietin fusion proteins in rat salivary glands. Biochem Biophys Res Commun 373:136-9

Zheng, Changyu; Vitolo, Joseph M; Zhang, Weitian et al. (2008) Extended transgene expression from a nonintegrating adenoviral vector containing retroviral elements. Mol Ther 16:1089-97

Samuni, Yuval; Cawley, Niamh X; Zheng, Changyu et al. (2008) Sorting behavior of a transgenic erythropoietin-growth hormone fusion protein in murine salivary glands. Hum Gene Ther 19:279-86

Nandula, Seshagiri R; Amarnath, Shoba; Molinolo, Alfredo et al. (2007) Female mice are more susceptible to developing inflammatory disorders due to impaired transforming growth factor beta signaling in salivary glands. Arthritis Rheum 56:1798-805

Yan, Xing; Voutetakis, Antonis; Zheng, Changyu et al. (2007) Sorting of transgenic secretory proteins in miniature pig parotid glands following adenoviral-mediated gene transfer. J Gene Med 9:779-87

Voutetakis, Antonis; Zheng, Changyu; Mineshiba, Fumi et al. (2007) Adeno-associated virus serotype 2-mediated gene transfer to the parotid glands of nonhuman primates. Hum Gene Ther 18:142-50

Baum, Bruce J (2007) Inadequate training in the biological sciences and medicine for dental students: an impending crisis for dentistry. J Am Dent Assoc 138:16, 18, 20 passim