Metabolism of Bone Cells in Vitro

Robey, P G.

Abstract

Bone cells (human and bovine) have been utilized to 1) study the biosynthesis of extracellular matrix proteins such as collagen, osteonectin, bone proteoglycan and other bone proteins, and alterations of matrix production in the disease Osteogenesis Imperfecta; 2) study the responsiveness of the cells to a variety of hormonal and pharmacological factors such as parathyroid hormone and 1,25 dihydroxy vitamin D3; 3) elucidate production and interaction of potential growth factors such as transforming growth factor-Beta, and 4) serve as a source of mRNA and DNA for studies of these proteins at the genomic level.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Dental & Craniofacial Research (NIDCR)
• Type: Intramural Research (Z01)
• Project #: 1Z01DE000380-03
• Application #: 3963735
• Support Year: 3
• Fiscal Year: 1986

Institution

• Name: Dental & Craniofacial Research
• Country: United States

Publications

Padmanabhan, Raji; Chen, Kevin G; Gillet, Jean-Pierre et al. (2012) Regulation and expression of the ATP-binding cassette transporter ABCG2 in human embryonic stem cells. Stem Cells 30:2175-87

Balakumaran, Arun; Robey, Pamela Gehron; Fedarko, Neal et al. (2010) Bone marrow microenvironment in myelomagenesis: its potential role in early diagnosis. Expert Rev Mol Diagn 10:465-80

Mazar, Julia; Thomas, Molly; Bezrukov, Ludmila et al. (2009) Cytotoxicity mediated by the Fas ligand (FasL)-activated apoptotic pathway in stem cells. J Biol Chem 284:22022-8

Pawelczyk, Edyta; Jordan, Elaine K; Balakumaran, Arun et al. (2009) In vivo transfer of intracellular labels from locally implanted bone marrow stromal cells to resident tissue macrophages. PLoS One 4:e6712

Inkson, Colette A; Ono, Mitsuaki; Kuznetsov, Sergei A et al. (2008) TGF-beta1 and WISP-1/CCN-4 can regulate each other's activity to cooperatively control osteoblast function. J Cell Biochem 104:1865-78

Tsutsui, T W; Riminucci, M; Holmbeck, Kenn et al. (2008) Development of craniofacial structures in transgenic mice with constitutively active PTH/PTHrP receptor. Bone 42:321-31

Pawelczyk, Edyta; Arbab, Ali S; Chaudhry, Aneeka et al. (2008) In vitro model of bromodeoxyuridine or iron oxide nanoparticle uptake by activated macrophages from labeled stem cells: implications for cellular therapy. Stem Cells 26:1366-75

Bianco, Paolo; Robey, Pamela Gehron; Simmons, Paul J (2008) Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell 2:313-9

Michienzi, Stefano; Cherman, Natasha; Holmbeck, Kenn et al. (2007) GNAS transcripts in skeletal progenitors: evidence for random asymmetric allelic expression of Gs alpha. Hum Mol Genet 16:1921-30

Cicconetti, Andrea; Sacchetti, Benedetto; Bartoli, Adriano et al. (2007) Human maxillary tuberosity and jaw periosteum as sources of osteoprogenitor cells for tissue engineering. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104:618.e1-12