Abstract

Bone cells derived from a variety of animal species including human, bovine, ovine and rodent, and of varying developmental ages, have been utilized to: 1) study the biosynthesis and deposition of extracellular matrix proteins such as collagen, osteonectin, bone proteoglycans and other bone proteins, and alterations of matrix production in disease states such as Osteogenesis Imperfecta; 2) study the responsiveness of the cells to a variety of pharmacological factors (such as calcium); 3) elucidate the production of and interaction with growth factors (such as TGF-beta, insulin-like growth factors and platelet-derived growth factors); 4) study the potential function of bone matrix proteins through the use of assays and the use of anti-sense DNA to inhibit specific protein synthesis; and 5) serve as a source of mRNA and DNA for studies of the proteins at the genomic level in collaboration with Drs. Marian F. Young and Larry W. Fisher.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Intramural Research (Z01)
Project #
1Z01DE000380-08
Application #
3854197
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
National Institute of Dental & Craniofacial Research
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Padmanabhan, Raji; Chen, Kevin G; Gillet, Jean-Pierre et al. (2012) Regulation and expression of the ATP-binding cassette transporter ABCG2 in human embryonic stem cells. Stem Cells 30:2175-87
Balakumaran, Arun; Robey, Pamela Gehron; Fedarko, Neal et al. (2010) Bone marrow microenvironment in myelomagenesis: its potential role in early diagnosis. Expert Rev Mol Diagn 10:465-80
Mazar, Julia; Thomas, Molly; Bezrukov, Ludmila et al. (2009) Cytotoxicity mediated by the Fas ligand (FasL)-activated apoptotic pathway in stem cells. J Biol Chem 284:22022-8
Pawelczyk, Edyta; Jordan, Elaine K; Balakumaran, Arun et al. (2009) In vivo transfer of intracellular labels from locally implanted bone marrow stromal cells to resident tissue macrophages. PLoS One 4:e6712
Inkson, Colette A; Ono, Mitsuaki; Kuznetsov, Sergei A et al. (2008) TGF-beta1 and WISP-1/CCN-4 can regulate each other's activity to cooperatively control osteoblast function. J Cell Biochem 104:1865-78
Tsutsui, T W; Riminucci, M; Holmbeck, Kenn et al. (2008) Development of craniofacial structures in transgenic mice with constitutively active PTH/PTHrP receptor. Bone 42:321-31
Pawelczyk, Edyta; Arbab, Ali S; Chaudhry, Aneeka et al. (2008) In vitro model of bromodeoxyuridine or iron oxide nanoparticle uptake by activated macrophages from labeled stem cells: implications for cellular therapy. Stem Cells 26:1366-75
Bianco, Paolo; Robey, Pamela Gehron; Simmons, Paul J (2008) Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell 2:313-9
Wallace, Joseph M; Rajachar, Rupak M; Allen, Matthew R et al. (2007) Exercise-induced changes in the cortical bone of growing mice are bone- and gender-specific. Bone 40:1120-7
Michienzi, Stefano; Cherman, Natasha; Holmbeck, Kenn et al. (2007) GNAS transcripts in skeletal progenitors: evidence for random asymmetric allelic expression of Gs alpha. Hum Mol Genet 16:1921-30

Showing the most recent 10 out of 71 publications