Ion Transport and Fluid Secretion in Salivary Glands

Turner, R J.

Abstract

Saliva is the principle protective agent for the mouth and thus is of primary importance to oral health maintenance. Perturbations of salivary secretory mechanisms can consequently lead to serious oral health problems. The objective of this project is to study the membrane and cellular processes which underlie the phenomenon of salivary fluid secretion and thus to contribute to our understanding of the fluid secretory process in normal and diseased states. Because similar secretory mechanisms are thought to be common to a number of other exocrine glands, this information should be of rather broad applicability and interest. During the present reporting period our specific areas of focus were the following. (1) Studies of the regulation of the rat parotid acinar Na-K-2Cl cotransporter by secretagogues and other stimuli were continued. (2) A polyclonal antibody against the rabbit parotid Na-K-2Cl cotransport protein was prepared and used to identify possible positive clones from a rabbit parotid cDNA library. (3) Investigations of the functional properties of salivary ducts were continued. In particular, the properties of an amiloride-sensitive sodium channel were characterized. (4) The effects of various attachment factors on the adhesion and spreading of the human salivary ductal cell line HSY were evaluated.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Dental & Craniofacial Research (NIDCR)
• Type: Intramural Research (Z01)
• Project #: 1Z01DE000415-08
• Application #: 3775666
• Support Year: 8
• Fiscal Year: 1993

Institution

• Name: National Institute of Dental & Craniofacial Research
• Country: United States

Publications

Nezu, Akihiro; Parvin, Most Nahid; Turner, R James (2009) A conserved hydrophobic tetrad near the C terminus of the secretory Na+-K+-2Cl- cotransporter (NKCC1) is required for its correct intracellular processing. J Biol Chem 284:6869-76

Nagy, Akos; Turner, R James (2007) The membrane integration of a naturally occurring alpha-helical hairpin. Biochem Biophys Res Commun 356:392-7

Parvin, Most Nahid; Gerelsaikhan, Tudevdagva; Turner, R James (2007) Regions in the cytosolic C-terminus of the secretory Na(+)-K(+)-2Cl(-) cotransporter NKCC1 are required for its homodimerization. Biochemistry 46:9630-7

Gerelsaikhan, Tudevdagva; Parvin, Most Nahid; Turner, R James (2006) Biogenesis and topology of the secretory Na+-K+-2Cl- cotransporter (NKCC1) studied in intact mammalian cells. Biochemistry 45:12060-7

Oh, Young S; Turner, R James (2006) Effect of gamma-secretase inhibitors on muscarinic receptor-mediated calcium signaling in human salivary epithelial cells. Am J Physiol Cell Physiol 291:C76-82

Oh, Young S; Turner, R James (2006) Protease digestion indicates that endogenous presenilin 1 is present in at least two physical forms. Biochem Biophys Res Commun 346:330-4

Oh, Young S; Turner, R James (2005) Evidence that the COOH terminus of human presenilin 1 is located in extracytoplasmic space. Am J Physiol Cell Physiol 289:C576-81

Oh, Young S; Turner, R James (2005) Topology of the C-terminal fragment of human presenilin 1. Biochemistry 44:11821-8

Tanimura, Akihiko; Nezu, Akihiro; Morita, Takao et al. (2004) Fluorescent biosensor for quantitative real-time measurements of inositol 1,4,5-trisphosphate in single living cells. J Biol Chem 279:38095-8

Dohke, Yoko; Oh, Young S; Ambudkar, Indu S et al. (2004) Biogenesis and topology of the transient receptor potential Ca2+ channel TRPC1. J Biol Chem 279:12242-8