Our goal is to understand the organization and control of taste signaling i.e. how individual pathways involved in taste transduction function and interact with each other. In the longer term we would like to define the components and the organization required for taste responses and to help elucidate the logic of taste coding. Specifically we would like to know what receptors mediate sweet and bitter taste; how tastant specificity and taste discrimination are accomplished; what topographic organization exists the various taste buds and papillae; and how the information is transmitted and encoded in the afferent nerves. We have focused on the isolation and characterization of genes encoding sweet, amino acid and bitter taste receptors that can be used to mark the cells, define the corresponding signaling pathways, dissect receptor specificity, generate topographic maps, and trace the respective neuronal connectivity circuits. We have identified and characterized two families of G-protein coupled receptors, T1Rs and T2Rs, that are expressed in distinct subsets of taste receptor cells and that include functionally validated sweet, amino acid and bitter taste receptors. The critical next steps in defining the logic of sweet, amino acid and bitter taste coding are to examine the physiology and connectivity pathways of T1R- and T2R-expressing cells in the various taste buds, and to study the impact of genetic ablation, or knockouts, of the different cells and receptor combinations.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Intramural Research (Z01)
Project #
1Z01DE000561-10
Application #
6663629
Study Section
(OIIB)
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
2002
Total Cost
Indirect Cost
Name
Dental & Craniofacial Research
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Nguyen, Minh Q; Zhou, Zhishang; Marks, Carolyn A et al. (2007) Prominent roles for odorant receptor coding sequences in allelic exclusion. Cell 131:1009-17
Huang, Angela L; Chen, Xiaoke; Hoon, Mark A et al. (2006) The cells and logic for mammalian sour taste detection. Nature 442:934-8
Chandrashekar, Jayaram; Hoon, Mark A; Ryba, Nicholas J P et al. (2006) The receptors and cells for mammalian taste. Nature 444:288-94
Mueller, Ken L; Hoon, Mark A; Erlenbach, Isolde et al. (2005) The receptors and coding logic for bitter taste. Nature 434:225-9
Zhang, Yifeng; Hoon, Mark A; Chandrashekar, Jayaram et al. (2003) Coding of sweet, bitter, and umami tastes: different receptor cells sharing similar signaling pathways. Cell 112:293-301
Zhao, Grace Q; Zhang, Yifeng; Hoon, Mark A et al. (2003) The receptors for mammalian sweet and umami taste. Cell 115:255-66
Nelson, Greg; Chandrashekar, Jayaram; Hoon, Mark A et al. (2002) An amino-acid taste receptor. Nature 416:199-202
Martini, S; Silvotti, L; Shirazi, A et al. (2001) Co-expression of putative pheromone receptors in the sensory neurons of the vomeronasal organ. J Neurosci 21:843-8
Nelson, G; Hoon, M A; Chandrashekar, J et al. (2001) Mammalian sweet taste receptors. Cell 106:381-90
Chandrashekar, J; Mueller, K L; Hoon, M A et al. (2000) T2Rs function as bitter taste receptors. Cell 100:703-11

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