Abstract

The Pilot Plant (Biotechnology Unit) combines several different types of activities. It is responsible for the large-scale production of bacteria, mammalian cells and biologically active compounds from various sources. Parallel to this activity, it conducts process development work associated with these preparations in order to be able to execute them efficiently. In addition, the unit carries on research work not necessarily associated with a current project, but work that has long-term implications for the unit's performance. During the last year, the unit carried out 123 different large-scale preparations, including microorganism growth in volumes from 10 to 300 liters, mammalian cell growth up to 50 liter volumes and processing of various biological materials. Development work was done in the growth optimization of various recombinant E. coli. An example is growth and recovery process for obtaining gr quantities of purified non-toxic exotoxin A lacking glutamic acid at position 553.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Intramural Research (Z01)
Project #
1Z01DK015500-30
Application #
3875545
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
30
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
U.S. National Inst Diabetes/Digst/Kidney
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Chill, Liat; Trinh, Loc; Azadi, Parastoo et al. (2009) Production, purification, and characterization of human alpha1 proteinase inhibitor from Aspergillus niger. Biotechnol Bioeng 102:828-44
Phue, Je-Nie; Kedem, Benjamin; Jaluria, Pratik et al. (2007) Evaluating microarrays using a semiparametric approach: application to the central carbon metabolism of Escherichia coli BL21 and JM109. Genomics 89:300-5
White, Jim F; Grodnitzky, Justin; Louis, John M et al. (2007) Dimerization of the class A G protein-coupled neurotensin receptor NTS1 alters G protein interaction. Proc Natl Acad Sci U S A 104:12199-204
Azurmendi, Hugo F; Vionnet, Justine; Wrightson, Lauren et al. (2007) Extracellular structure of polysialic acid explored by on cell solution NMR. Proc Natl Acad Sci U S A 104:11557-61
Karnaukhova, Elena; Ophir, Yakir; Trinh, Loc et al. (2007) Expression of human alpha1-proteinase inhibitor in Aspergillus niger. Microb Cell Fact 6:34
Jaluria, Pratik; Betenbaugh, Michael; Konstantopoulos, Konstantinos et al. (2007) Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells. Metab Eng 9:241-51
Kim, Chul-Hee; Pennisi, Patricia; Zhao, Hong et al. (2006) MKR mice are resistant to the metabolic actions of both insulin and adiponectin: discordance between insulin resistance and adiponectin responsiveness. Am J Physiol Endocrinol Metab 291:E298-305
Bell, Jessica K; Botos, Istvan; Hall, Pamela R et al. (2006) The molecular structure of the TLR3 extracellular domain. J Endotoxin Res 12:375-8
Trinh, Loc; Phue, Je-Nie; Jaluria, Pratik et al. (2006) Screen-less expanded bed column: new approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris. Biotechnol Lett 28:951-8
Jin, Zhigang; Bohach, Gregory A; Shiloach, Joseph et al. (2005) Conjugates of group A and W135 capsular polysaccharides of neisseria meningitidis bound to recombinant Staphylococcus aureus enterotoxin C1: preparation, physicochemical characterization, and immunological properties in mice. Infect Immun 73:7887-93

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