Large-Scale Processing of Biological Material

Shiloach, J.

Abstract

The Pilot Plant (Biotechnology Unit) combines several different types of activities. It is responsible for the large-scale production of bacteria, mammalian cells and biologically active compounds from various sources. Parallel to this activity, it conducts Process development work associated with these preparations in order to be able to execute them efficiently. In addition, the unit carries on research work not necessarily associated with a current project, but work that has long-term implications for the Unit's performance. During the last year, the Unit produced 185 different large-scale preparations, including microorganism grown in volumes from 10 to 300 liters, mammalian cell growth up to 50 liter volumes and processing of various biological materials. Process development work was done on large-scale purification of two proteins needed for the first phase of clinical trials. One is a modified form of Pseudomonas aeruginosa exotoxin A from recombinant E. coli and the second is S antigen from bovine retinas. Research was conducted on developing an adaptive control strategy for maintaining the dissolved oxygen level in the aerobic growth of microorganisms.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Intramural Research (Z01)
• Project #: 1Z01DK015500-32
• Application #: 3854538
• Support Year: 32
• Fiscal Year: 1991

Institution

• Name: National Institute of Diabetes and Digestive and Kidney Diseases
• Country: United States

Publications

Chill, Liat; Trinh, Loc; Azadi, Parastoo et al. (2009) Production, purification, and characterization of human alpha1 proteinase inhibitor from Aspergillus niger. Biotechnol Bioeng 102:828-44

Phue, Je-Nie; Kedem, Benjamin; Jaluria, Pratik et al. (2007) Evaluating microarrays using a semiparametric approach: application to the central carbon metabolism of Escherichia coli BL21 and JM109. Genomics 89:300-5

White, Jim F; Grodnitzky, Justin; Louis, John M et al. (2007) Dimerization of the class A G protein-coupled neurotensin receptor NTS1 alters G protein interaction. Proc Natl Acad Sci U S A 104:12199-204

Azurmendi, Hugo F; Vionnet, Justine; Wrightson, Lauren et al. (2007) Extracellular structure of polysialic acid explored by on cell solution NMR. Proc Natl Acad Sci U S A 104:11557-61

Karnaukhova, Elena; Ophir, Yakir; Trinh, Loc et al. (2007) Expression of human alpha1-proteinase inhibitor in Aspergillus niger. Microb Cell Fact 6:34

Jaluria, Pratik; Betenbaugh, Michael; Konstantopoulos, Konstantinos et al. (2007) Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells. Metab Eng 9:241-51

Kim, Chul-Hee; Pennisi, Patricia; Zhao, Hong et al. (2006) MKR mice are resistant to the metabolic actions of both insulin and adiponectin: discordance between insulin resistance and adiponectin responsiveness. Am J Physiol Endocrinol Metab 291:E298-305

Bell, Jessica K; Botos, Istvan; Hall, Pamela R et al. (2006) The molecular structure of the TLR3 extracellular domain. J Endotoxin Res 12:375-8

Trinh, Loc; Phue, Je-Nie; Jaluria, Pratik et al. (2006) Screen-less expanded bed column: new approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris. Biotechnol Lett 28:951-8

Jin, Zhigang; Bohach, Gregory A; Shiloach, Joseph et al. (2005) Conjugates of group A and W135 capsular polysaccharides of neisseria meningitidis bound to recombinant Staphylococcus aureus enterotoxin C1: preparation, physicochemical characterization, and immunological properties in mice. Infect Immun 73:7887-93