The Biotechnology Unit is responsible for: (a) Large-scale production of procaryotes (bacteria), and eucaryotes (mammalian cells, insect cells); (b) Large-scale recovery and purification of biologically active compounds (proteins, polysaccharides, etc.) from various sources; (c) Process development work associated with (1) bacterial growth, (2) eucaryotic cell growth, and (3) extraction and purification of biologically active compounds, especially proteins. The process development work is conducted to develop a procedure suitable for large-scale preparation and production of material suitable for clinical trials; and (d) Research and development work not necessarily linked to a current process development project, but work that has long-term implications for fermentation processes and protein purification. During the last year the Unit performed 212 different large-scale preparations, including microorganisms (especially E. coli-carrying recombinant DNA) grown in volumes ranging from 5 to 300 liters, eucaryotic cells grown in volume up to 50 liters and processing of various biological materials. Special effort was devoted to the development of a suitable process for the extraction and purification of large amounts of two proteins needed for clinical trials. The first is S antigen from the human retina and the second is a modified toxic version of Pseudomonas aeruginosa exotoxin A.

Project Start
Project End
Budget Start
Budget End
Support Year
33
Fiscal Year
1992
Total Cost
Indirect Cost
City
State
Country
United States
Zip Code
Chill, Liat; Trinh, Loc; Azadi, Parastoo et al. (2009) Production, purification, and characterization of human alpha1 proteinase inhibitor from Aspergillus niger. Biotechnol Bioeng 102:828-44
Phue, Je-Nie; Kedem, Benjamin; Jaluria, Pratik et al. (2007) Evaluating microarrays using a semiparametric approach: application to the central carbon metabolism of Escherichia coli BL21 and JM109. Genomics 89:300-5
White, Jim F; Grodnitzky, Justin; Louis, John M et al. (2007) Dimerization of the class A G protein-coupled neurotensin receptor NTS1 alters G protein interaction. Proc Natl Acad Sci U S A 104:12199-204
Azurmendi, Hugo F; Vionnet, Justine; Wrightson, Lauren et al. (2007) Extracellular structure of polysialic acid explored by on cell solution NMR. Proc Natl Acad Sci U S A 104:11557-61
Karnaukhova, Elena; Ophir, Yakir; Trinh, Loc et al. (2007) Expression of human alpha1-proteinase inhibitor in Aspergillus niger. Microb Cell Fact 6:34
Jaluria, Pratik; Betenbaugh, Michael; Konstantopoulos, Konstantinos et al. (2007) Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells. Metab Eng 9:241-51
Kim, Chul-Hee; Pennisi, Patricia; Zhao, Hong et al. (2006) MKR mice are resistant to the metabolic actions of both insulin and adiponectin: discordance between insulin resistance and adiponectin responsiveness. Am J Physiol Endocrinol Metab 291:E298-305
Bell, Jessica K; Botos, Istvan; Hall, Pamela R et al. (2006) The molecular structure of the TLR3 extracellular domain. J Endotoxin Res 12:375-8
Trinh, Loc; Phue, Je-Nie; Jaluria, Pratik et al. (2006) Screen-less expanded bed column: new approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris. Biotechnol Lett 28:951-8
Jin, Zhigang; Bohach, Gregory A; Shiloach, Joseph et al. (2005) Conjugates of group A and W135 capsular polysaccharides of neisseria meningitidis bound to recombinant Staphylococcus aureus enterotoxin C1: preparation, physicochemical characterization, and immunological properties in mice. Infect Immun 73:7887-93

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