Structural Studies Of AIDS Proteins By NMR

Gronenborn, Angela M.

Abstract

Work has been carried out on a number of structural problems related to proteins derived from retroviruses and of relevance to the pathogenesis and clinical manifestation of AIDS. These include the potent anti HIV protein cyanovirin-N, variants thereof and HIV-1 protease, in particular a monomeric form of this enzyme. In addition, methodological advances have been made in devising new pulse sequences to easily measure residual dipolar couplings of aligned biological macromolecules in the magnetic field.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Intramural Research (Z01)
• Project #: 1Z01DK029022-16
• Application #: 6983738
• Study Section: (LCP)
• Support Year: 16
• Fiscal Year: 2004

Institution

• Name: U.S. National Inst Diabetes/Digst/Kidney
• Country: United States

Publications

Barrientos, Laura G; Gronenborn, Angela M (2005) The highly specific carbohydrate-binding protein cyanovirin-N: structure, anti-HIV/Ebola activity and possibilities for therapy. Mini Rev Med Chem 5:21-31

Ding, Keyang; Gronenborn, Angela M (2004) Sensitivity-enhanced IPAP experiments for measuring one-bond 13C'-13Calpha and 13Calpha-1Halpha residual dipolar couplings in proteins. J Magn Reson 167:253-8

Barrientos, Laura G; Lasala, Fatima; Delgado, Rafael et al. (2004) Flipping the switch from monomeric to dimeric CV-N has little effect on antiviral activity. Structure 12:1799-807

Ding, Keyang; Gronenborn, Angela M (2004) Protein Backbone 1H(N)-13Calpha and 15N-13Calpha residual dipolar and J couplings: new constraints for NMR structure determination. J Am Chem Soc 126:6232-3

Ishima, Rieko; Torchia, Dennis A; Lynch, Shannon M et al. (2003) Solution structure of the mature HIV-1 protease monomer: insight into the tertiary fold and stability of a precursor. J Biol Chem 278:43311-9

Louis, John M; Ishima, Rieko; Nesheiwat, Issa et al. (2003) Revisiting monomeric HIV-1 protease. Characterization and redesign for improved properties. J Biol Chem 278:6085-92

Katoh, Etsuko; Louis, John M; Yamazaki, Toshimasa et al. (2003) A solution NMR study of the binding kinetics and the internal dynamics of an HIV-1 protease-substrate complex. Protein Sci 12:1376-85

Barrientos, Laura G; Louis, John M; Ratner, Daniel M et al. (2003) Solution structure of a circular-permuted variant of the potent HIV-inactivating protein cyanovirin-N: structural basis for protein stability and oligosaccharide interaction. J Mol Biol 325:211-23

Ding, Keyang; Gronenborn, Angela M (2003) Sensitivity-enhanced 2D IPAP, TROSY-anti-TROSY, and E.COSY experiments: alternatives for measuring dipolar 15N-1HN couplings. J Magn Reson 163:208-14

Barrientos, Laura G; O'Keefe, Barry R; Bray, Mike et al. (2003) Cyanovirin-N binds to the viral surface glycoprotein, GP1,2 and inhibits infectivity of Ebola virus. Antiviral Res 58:47-56