Research in this laboratory is centered around solution studies on the structure of proteins, protein-protein complexes and protein-nucleic acid complexes using multidimensional NMR spectroscopy, and on the development and application of novel NMR and computational methods to aid in these studies. ? Over the last year we have developed several new multidimensional pulse sequences to derive assignments using direct-carbon detect methods; multidimensional sequences to detect arginine guanidino and lysine NH3 groups; new approaches to docking protein-protein complexes based on very sparse intermolecular NOE data; and we have solved the structures of several large protein-protein complexes, including a phosphoryl transfer complexes of enzymes IIAMannitol and IIBMannitol, and IIAmannose and IIBMannose from the bacterial phosphoryl transfer system and the complex of the barrier-to-autointegration factor with emerin.
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