Enzyme Structure

Davies, D R.

Abstract

1) Several data sets of the tryptophan synthase crystals from Salmonella typhimurium have been refined. The refined structures have confirmed the original observation at lower resolution of a tunnel for the passage of indole generated in the first reaction to the active site of the second enzyme. Mutants are being examined with a view to elucidating the dynamic nature of this tunnel and of the site interaction in the complex. 2) Inhibitor complexes of rhizopus pepsin have been analyzed by X- ray diffraction with a view to obtaining more information concerning the mechanism of action. 3) A method has been developed for the joint refinement of structures determined by X-ray diffraction and by NMR. 4) Crystals have been obtained for the complex of a Y-box protein from Xenopus oocytes with a DNA oligomer.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Intramural Research (Z01)
• Project #: 1Z01DK034002-27
• Application #: 3854856
• Support Year: 27
• Fiscal Year: 1991

Institution

• Name: National Institute of Diabetes and Digestive and Kidney Diseases
• Country: United States

Publications

Liu, Lin; Botos, Istvan; Wang, Yan et al. (2008) Structural basis of toll-like receptor 3 signaling with double-stranded RNA. Science 320:379-81

Leonard, Joshua N; Ghirlando, Rodolfo; Askins, Janine et al. (2008) The TLR3 signaling complex forms by cooperative receptor dimerization. Proc Natl Acad Sci U S A 105:258-63

Bell, Jessica K; Askins, Janine; Hall, Pamela R et al. (2006) The dsRNA binding site of human Toll-like receptor 3. Proc Natl Acad Sci U S A 103:8792-7

Cohen, Gerson H; Silverton, Enid W; Padlan, Eduardo A et al. (2005) Water molecules in the antibody-antigen interface of the structure of the Fab HyHEL-5-lysozyme complex at 1.7 A resolution: comparison with results from isothermal titration calorimetry. Acta Crystallogr D Biol Crystallogr 61:628-33

Bell, Jessica K; Botos, Istvan; Hall, Pamela R et al. (2005) The molecular structure of the Toll-like receptor 3 ligand-binding domain. Proc Natl Acad Sci U S A 102:10976-80

Bell, Jessica K; Mullen, Gregory E D; Leifer, Cynthia A et al. (2003) Leucine-rich repeats and pathogen recognition in Toll-like receptors. Trends Immunol 24:528-33

Hickman, A B; Li, Y; Mathew, S V et al. (2000) Unexpected structural diversity in DNA recombination: the restriction endonuclease connection. Mol Cell 5:1025-34

Fan, Y X; McPhie, P; Miles, E W (2000) Regulation of tryptophan synthase by temperature, monovalent cations, and an allosteric ligand. Evidence from Arrhenius plots, absorption spectra, and primary kinetic isotope effects. Biochemistry 39:4692-703

Fan, Y X; McPhie, P; Miles, E W (2000) Thermal repair of tryptophan synthase mutations in a regulatory intersubunit salt bridge. Evidence from arrhenius plots, absorption spectra, and primary kinetic isotope effects. J Biol Chem 275:20302-7

Jhee, K H; McPhie, P; Miles, E W (2000) Yeast cystathionine beta-synthase is a pyridoxal phosphate enzyme but, unlike the human enzyme, is not a heme protein. J Biol Chem 275:11541-4