1) Crystals of the Rnase H from HIV have been investigated. The crystals diffract to about 3.5A resolution. Many heavy atom derivatives have been examined in order to provide an interpretable electron density map. 2) Crystals obtained of the OKT4A/CD4 complex have been grown to X-ray dimensions. Examination of these crystals showed that they do not diffract to atomic resolution. The crystallization conditions for the HIV integrase protein are being investigated.

Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
1991
Total Cost
Indirect Cost
City
State
Country
United States
Zip Code
Baxa, Ulrich; Cheng, Naiqian; Winkler, Dennis C et al. (2005) Filaments of the Ure2p prion protein have a cross-beta core structure. J Struct Biol 150:170-9
Davies, David (2005) A quiet life with proteins. Annu Rev Biophys Biomol Struct 34:1-20
Chiu, Thang K; Kubelka, Jan; Herbst-Irmer, Regine et al. (2005) High-resolution x-ray crystal structures of the villin headpiece subdomain, an ultrafast folding protein. Proc Natl Acad Sci U S A 102:7517-22
Chiu, Thang K; Davies, David R (2004) Structure and function of HIV-1 integrase. Curr Top Med Chem 4:965-77
Umland, Timothy C; Wolff, Edith C; Park, Myung Hee et al. (2004) A new crystal structure of deoxyhypusine synthase reveals the configuration of the active enzyme and of an enzyme.NAD.inhibitor ternary complex. J Biol Chem 279:28697-705