In 1996 a workshop on Measurement of Oxidative Stress in Humans was held at NIEHS. There was a general consensus that it would be valuable to mount a study comparing various markers of oxidative stress measured from the same sample. One such marker is F2a-isoprostane which is quantitated by NICI-MS. Isoprostanes are prostaglandin-like compounds that are produced as a result of the chemical rather than enzymatic oxidation of arachidonic acid in membrane phospholipids. The isoprostanes have been shown to possess potent biological activity, and a number of studies have shown them to be accurate markers of lipid peroxidation in animal models of oxidative stress. The 8-iso-F2a-isoprostane has been the specific isomer quantified in most studies. Although there is recent evidence for some cyclooxygenase catalyzed formation of this compound, the contribution of the enzymatic pathway has appeared to be minor and primarily the result of oxidation of free lipid rather than membrane-bound lipid. The standard method for analyzing this compound is by GC/MS of the pentafluorobenzyl ester trimethylsilyl derivative under negative ion chemical ionization conditions. Our role in this study has been to establish methodology in-house for the measurement of isoprostanes in physiological samples and to provide this capability in collaboration with other groups at NIEHS/NIH. A number of other studies involving measurement of isoprostanes as an indicator of oxidative stress have also been initiated. Women's Alcohol Study P. Taylor (NCI)/D. Baer, J. Judd (USDA)Correlation of O.S. with mutations (T. Devereaux, LMC)identified mutations in chemically induced mouse hepatocellular adenomas and carcinomas hypothesize mutations are the result of indirect DNA damage, possibly from O.S.?Mechanisms of oxidative damage group - A cross-laboratory group interested in comparing various markers of non-genotoxic damage to tissues as a result of treatment with specific toxic substances. The goal is to compare which types of toxins initiate which types of damage to provide a better mechanistic understanding of their toxicity. We are providing isoprostane analyses for this study.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Intramural Research (Z01)
Project #
1Z01ES050167-03
Application #
6432366
Study Section
(LSB)
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2000
Total Cost
Indirect Cost
Name
U.S. National Inst of Environ Hlth Scis
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Cheng, Jennifer; Dackor, Ryan T; Bradbury, J Alyce et al. (2016) Contribution of alveolar type II cell-derived cyclooxygenase-2 to basal airway function, lung inflammation, and lung fibrosis. FASEB J 30:160-73
Cheng, Jennifer; Edin, Matthew L; Hoopes, Samantha L et al. (2014) Vascular characterization of mice with endothelial expression of cytochrome P450 4F2. FASEB J 28:2915-31
Takeda, Yukimasa; Kang, Hong Soon; Lih, Fred B et al. (2014) Retinoid acid-related orphan receptor ?, ROR?, participates in diurnal transcriptional regulation of lipid metabolic genes. Nucleic Acids Res 42:10448-59
Zha, Weibin; Edin, Matthew L; Vendrov, Kimberly C et al. (2014) Functional characterization of cytochrome P450-derived epoxyeicosatrienoic acids in adipogenesis and obesity. J Lipid Res 55:2124-36
Schuck, Robert N; Zha, Weibin; Edin, Matthew L et al. (2014) The cytochrome P450 epoxygenase pathway regulates the hepatic inflammatory response in fatty liver disease. PLoS One 9:e110162
Theken, Katherine N; Schuck, Robert N; Edin, Matthew L et al. (2012) Evaluation of cytochrome P450-derived eicosanoids in humans with stable atherosclerotic cardiovascular disease. Atherosclerosis 222:530-6
Panigrahy, Dipak; Edin, Matthew L; Lee, Craig R et al. (2012) Epoxyeicosanoids stimulate multiorgan metastasis and tumor dormancy escape in mice. J Clin Invest 122:178-91
Panigrahy, Dipak; Greene, Emily R; Pozzi, Ambra et al. (2011) EET signaling in cancer. Cancer Metastasis Rev 30:525-40
Deng, Yangmei; Edin, Matthew L; Theken, Katherine N et al. (2011) Endothelial CYP epoxygenase overexpression and soluble epoxide hydrolase disruption attenuate acute vascular inflammatory responses in mice. FASEB J 25:703-13
Lee, Craig R; Imig, John D; Edin, Matthew L et al. (2010) Endothelial expression of human cytochrome P450 epoxygenases lowers blood pressure and attenuates hypertension-induced renal injury in mice. FASEB J 24:3770-81

Showing the most recent 10 out of 20 publications