We reported previously that several mutations that abolish either of the two mechanisms of replication repair are general mutator mutations that sometimes promote templated mutations particularly strongly. Thus, defects in replication repair somehow promote template switching to an inappropriate template. We have now tested predictions about the properties of mutants carrying mutational blocks either in both pathways, or in both steps of one of the pathways. We are also testing whether DNA damage can promote templated mutagenesis.
| Schultz Jr, Gary E; Drake, John W (2008) Templated mutagenesis in bacteriophage T4 involving imperfect direct or indirect sequence repeats. Genetics 178:661-73 |
| Schultz Jr, Gary E; Carver, Geraldine T; Drake, John W (2006) A role for replication repair in the genesis of templated mutations. J Mol Biol 358:963-73 |
| Kadyrov, Farid A; Drake, John W (2004) UvsX recombinase and Dda helicase rescue stalled bacteriophage T4 DNA replication forks in vitro. J Biol Chem 279:35735-40 |
| Kadyrov, Farid A; Drake, John W (2003) Properties of bacteriophage T4 proteins deficient in replication repair. J Biol Chem 278:25247-55 |
| Kadyrov, Farid A; Drake, John W (2002) Characterization of DNA synthesis catalyzed by bacteriophage T4 replication complexes reconstituted on synthetic circular substrates. Nucleic Acids Res 30:4387-97 |
| Kadyrov, F A; Drake, J W (2001) Conditional coupling of leading-strand and lagging-strand DNA synthesis at bacteriophage T4 replication forks. J Biol Chem 276:29559-66 |
