We have identified a new class of voltage-activated calcium channels with low thresholds and slow inactivation that are stimulated by protein kinase C in immortalized LHRH neurons. These channels are similar in amplitude to other voltage-activated calcium channels, but they are insensitive to block by conus toxins. We have also shown that large- conductance, calcium- and voltage-activated potassium channels in immortalized lactotrophs are inhibited by protein kinase C, which represents the first molecular mechanism linking phospholipase C activation to sustained increases in electrical excitability. We have extended our studies of potassium channel modulation by protein phosphatases to single channels in cell-free patches of membrane, and initiated experiments to investigate the role of hormonally activated protein phosphatases, elucidated by earlier studies from this laboratory, in neuronal degeneration associated with AIDS and Alzheimer's disease.
| Lucas, Sarah J; Armstrong, David L (2015) Protein phosphatase modulation of somatostatin receptor signaling in the mouse hippocampus. Neuropharmacology 99:232-41 |
| Chatterjee, Anindya; Wang, Ling; Armstrong, David L et al. (2010) Activated Rac1 GTPase translocates protein phosphatase 5 to the cell membrane and stimulates phosphatase activity in vitro. J Biol Chem 285:3872-82 |
| Armstrong, David L; Erxleben, Christian; White, Jody A (2010) Patch clamp methods for studying calcium channels. Methods Cell Biol 99:183-97 |
| Sanchez-Ortiz, Efrain; Hahm, Byoung Kwon; Armstrong, David L et al. (2009) Protein phosphatase 5 protects neurons against amyloid-beta toxicity. J Neurochem 111:391-402 |
| Liao, Yanhong; Erxleben, Christian; Yildirim, Eda et al. (2007) Orai proteins interact with TRPC channels and confer responsiveness to store depletion. Proc Natl Acad Sci U S A 104:4682-7 |
| Erxleben, Christian; Liao, Yanhong; Gentile, Saverio et al. (2006) Cyclosporin and Timothy syndrome increase mode 2 gating of CaV1.2 calcium channels through aberrant phosphorylation of S6 helices. Proc Natl Acad Sci U S A 103:3932-7 |
| Gentile, Saverio; Darden, Thomas; Erxleben, Christian et al. (2006) Rac GTPase signaling through the PP5 protein phosphatase. Proc Natl Acad Sci U S A 103:5202-6 |
| Tian, Lijun; Coghill, Lorraine S; MacDonald, Stephen H-F et al. (2003) Leucine zipper domain targets cAMP-dependent protein kinase to mammalian BK channels. J Biol Chem 278:8669-77 |
| Storey, Nina M; Gomez-Angelats, Mireia; Bortner, Carl D et al. (2003) Stimulation of Kv1.3 potassium channels by death receptors during apoptosis in Jurkat T lymphocytes. J Biol Chem 278:33319-26 |
| Erxleben, Christian; Gomez-Alegria, Claudio; Darden, Thomas et al. (2003) Modulation of cardiac Ca(V)1.2 channels by dihydropyridine and phosphatase inhibitor requires Ser-1142 in the domain III pore loop. Proc Natl Acad Sci U S A 100:2929-34 |
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