Vitamin a and Ocular Tissues

Wiggert, B.

Abstract

Interphotoreceptor retinoid-Binding protein (IRBP) was shown by enzyme-linked immunosorbent assay (ELISA) to be absent from monkey liver, testes, lens and cornea. IRBP was present in cerebral cortex, pineal, vitreous and aqueous humor. These results were confirmed by Western Blot. IRBP was localized to rod photoreceptor cells in monkey retina by means of immunoelectronmicroscopy. Rats immunized with purified IRBP developed intraocular inflammatory changes and pineal gland inflammatory changes. Incubation of Y-79 retinoblastoma cells grown in monolayer tissue culture with radiolabeled precursors showed IRBP to be synthesized and secreted by these cells. IRBP synthesis was markedly increased by butyrate treatment. Purified monkey IRBP was found to contain 6.64 mol. of endogenous fatty acids per mol. protein. 60% of the total fatty acid was physically attached; the remaining 40% was covalently bound. Monkey, human and bovine IRBP were compared by analytical peptide mapping which showed monkey and human IRBP to be very similar and distinct from bovine IRBP. Tryptic peptides from bovine IRBP were isolated by reversed-phased high performance liquid chromatography (HPLC) and sequenced. One such peptide was used to confirm the identity of an IRBP clone.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Eye Institute (NEI)
• Type: Intramural Research (Z01)
• Project #: 1Z01EY000070-08
• Application #: 4693325
• Support Year: 8
• Fiscal Year: 1985

Institution

• Name: U.S. National Eye Institute
• Country: United States

Publications

Cortes, Lizette M; Mattapallil, Mary J; Silver, Phyllis B et al. (2008) Repertoire analysis and new pathogenic epitopes of IRBP in C57BL/6 (H-2b) and B10.RIII (H-2r) mice. Invest Ophthalmol Vis Sci 49:1946-56

Wu, Qingqing; Blakeley, Lorie R; Cornwall, M Carter et al. (2007) Interphotoreceptor retinoid-binding protein is the physiologically relevant carrier that removes retinol from rod photoreceptor outer segments. Biochemistry 46:8669-79

Duncan, Todd; Wiggert, Barbara; Whittaker, Noel et al. (2006) Effect of visible light on normal and P23H-3 transgenic rat retinas: characterization of a novel retinoic acid derivative present in the P23H-3 retina. Photochem Photobiol 82:741-5

Ala-Laurila, Petri; Kolesnikov, Alexander V; Crouch, Rosalie K et al. (2006) Visual cycle: Dependence of retinol production and removal on photoproduct decay and cell morphology. J Gen Physiol 128:153-69

Kutty, R Krishnan; Chen, Shanyi; Samuel, William et al. (2006) Cell density-dependent nuclear/cytoplasmic localization of NORPEG (RAI14) protein. Biochem Biophys Res Commun 345:1333-41

Kutty, R Krishnan; Samuel, William; Chen, Shanyi et al. (2006) Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Muller and ganglion cells. Neurosci Lett 404:294-8

Qtaishat, Nasser M; Wiggert, Barbara; Pepperberg, David R (2005) Interphotoreceptor retinoid-binding protein (IRBP) promotes the release of all-trans retinol from the isolated retina following rhodopsin bleaching illumination. Exp Eye Res 81:455-63

Tsina, Efthymia; Chen, Chunhe; Koutalos, Yiannis et al. (2004) Physiological and microfluorometric studies of reduction and clearance of retinal in bleached rod photoreceptors. J Gen Physiol 124:429-43

Cornwall, M Carter; Tsina, Efthymia; Crouch, Rosalie K et al. (2003) Regulation of the visual cycle: retinol dehydrogenase and retinol fluorescence measurements in vertebrate retina. Adv Exp Med Biol 533:353-60

Wang, Peng; Sun, Shu-Hui; Silver, Phyllis B et al. (2003) Methimazole protects from experimental autoimmune uveitis (EAU) by inhibiting antigen presenting cell function and reducing antigen priming. J Leukoc Biol 73:57-64