Abstract

An enzyme-linked immunosorbent assay (ELISA) for monkey and human Interphoto-receptor Retinoid-Binding Protein (IRBP) was used to quantitate IRBP in normal and diseased human retinas and retinoblastoma tumors. IRBP levels were uniformly low in retinas from human cases of hereditary retinal degenerations even in areas in which photoreceptors remained. IRBP was present in several retinoblastoma tumors examined. The amino terminal sequences of monkey and bovine IRBPs were extended to over 30 residues each. The major monkey sequence had an additional 5 amino acid residues at its amino terminus not observed with bovine IRBP, although the sequences showed extensive homology. The amino terminal sequence of human IRBP was identical to that of the monkey, and the two sequences were present in equal amount.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Intramural Research (Z01)
Project #
1Z01EY000070-09
Application #
3965346
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
U.S. National Eye Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Cortes, Lizette M; Mattapallil, Mary J; Silver, Phyllis B et al. (2008) Repertoire analysis and new pathogenic epitopes of IRBP in C57BL/6 (H-2b) and B10.RIII (H-2r) mice. Invest Ophthalmol Vis Sci 49:1946-56
Wu, Qingqing; Blakeley, Lorie R; Cornwall, M Carter et al. (2007) Interphotoreceptor retinoid-binding protein is the physiologically relevant carrier that removes retinol from rod photoreceptor outer segments. Biochemistry 46:8669-79
Duncan, Todd; Wiggert, Barbara; Whittaker, Noel et al. (2006) Effect of visible light on normal and P23H-3 transgenic rat retinas: characterization of a novel retinoic acid derivative present in the P23H-3 retina. Photochem Photobiol 82:741-5
Ala-Laurila, Petri; Kolesnikov, Alexander V; Crouch, Rosalie K et al. (2006) Visual cycle: Dependence of retinol production and removal on photoproduct decay and cell morphology. J Gen Physiol 128:153-69
Kutty, R Krishnan; Chen, Shanyi; Samuel, William et al. (2006) Cell density-dependent nuclear/cytoplasmic localization of NORPEG (RAI14) protein. Biochem Biophys Res Commun 345:1333-41
Kutty, R Krishnan; Samuel, William; Chen, Shanyi et al. (2006) Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Muller and ganglion cells. Neurosci Lett 404:294-8
Qtaishat, Nasser M; Wiggert, Barbara; Pepperberg, David R (2005) Interphotoreceptor retinoid-binding protein (IRBP) promotes the release of all-trans retinol from the isolated retina following rhodopsin bleaching illumination. Exp Eye Res 81:455-63
Tsina, Efthymia; Chen, Chunhe; Koutalos, Yiannis et al. (2004) Physiological and microfluorometric studies of reduction and clearance of retinal in bleached rod photoreceptors. J Gen Physiol 124:429-43
Cornwall, M Carter; Tsina, Efthymia; Crouch, Rosalie K et al. (2003) Regulation of the visual cycle: retinol dehydrogenase and retinol fluorescence measurements in vertebrate retina. Adv Exp Med Biol 533:353-60
Wang, Peng; Sun, Shu-Hui; Silver, Phyllis B et al. (2003) Methimazole protects from experimental autoimmune uveitis (EAU) by inhibiting antigen presenting cell function and reducing antigen priming. J Leukoc Biol 73:57-64

Showing the most recent 10 out of 16 publications