Abstract

In the intact eye of the developing rd/rd, +/+ mutant mouse, interphotoreceptor-binding protein (IRBP), which is normally located extracellularly in the interphotoreceptor matrix, remains intracellular. However, when removed from the eye and placed in short-term organ culture, retinas from these mutant mice demonstrate the capacity to synthesize and secrete IRBP normally until postnatal day 11 - 12. There may then be regulatory factors which control IRBP secretion in the intact eye. In studies of the physiological role of IRBP in the normal retina using the toad (Bufo marinus) eye-cup preparation, IRBP was shown to be capable of promoting regeneration of rhodopsin in bleached ROS. Serum albumin, however, did not promote regeneration. In addition, it was shown that 11-cis retinal was removed from the RPE by IRBP but not by serum albumin. It appears, then, that IRBP plays an active role in the vision process. In initial studies on IRBP in mouse eyes inoculated with a murine coronavirus, there was a highly significant decrease in IRBP by day 3 following inoculation. In addition, IRBP was no longer restricted to its normal location in the interphotoreceptor matrix but had diffused in the direction of the vitreous, reaching as far as the inner nuclear layer.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Intramural Research (Z01)
Project #
1Z01EY000070-13
Application #
3877027
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
1990
Total Cost
Indirect Cost

  Institution

Name
U.S. National Eye Institute
Department
Type
DUNS #
City
State
Country
United States
Zip Code

  Publications

Cortes, Lizette M; Mattapallil, Mary J; Silver, Phyllis B et al. (2008) Repertoire analysis and new pathogenic epitopes of IRBP in C57BL/6 (H-2b) and B10.RIII (H-2r) mice. Invest Ophthalmol Vis Sci 49:1946-56
Wu, Qingqing; Blakeley, Lorie R; Cornwall, M Carter et al. (2007) Interphotoreceptor retinoid-binding protein is the physiologically relevant carrier that removes retinol from rod photoreceptor outer segments. Biochemistry 46:8669-79
Duncan, Todd; Wiggert, Barbara; Whittaker, Noel et al. (2006) Effect of visible light on normal and P23H-3 transgenic rat retinas: characterization of a novel retinoic acid derivative present in the P23H-3 retina. Photochem Photobiol 82:741-5
Ala-Laurila, Petri; Kolesnikov, Alexander V; Crouch, Rosalie K et al. (2006) Visual cycle: Dependence of retinol production and removal on photoproduct decay and cell morphology. J Gen Physiol 128:153-69
Kutty, R Krishnan; Chen, Shanyi; Samuel, William et al. (2006) Cell density-dependent nuclear/cytoplasmic localization of NORPEG (RAI14) protein. Biochem Biophys Res Commun 345:1333-41
Kutty, R Krishnan; Samuel, William; Chen, Shanyi et al. (2006) Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Muller and ganglion cells. Neurosci Lett 404:294-8
Qtaishat, Nasser M; Wiggert, Barbara; Pepperberg, David R (2005) Interphotoreceptor retinoid-binding protein (IRBP) promotes the release of all-trans retinol from the isolated retina following rhodopsin bleaching illumination. Exp Eye Res 81:455-63
Tsina, Efthymia; Chen, Chunhe; Koutalos, Yiannis et al. (2004) Physiological and microfluorometric studies of reduction and clearance of retinal in bleached rod photoreceptors. J Gen Physiol 124:429-43
Cornwall, M Carter; Tsina, Efthymia; Crouch, Rosalie K et al. (2003) Regulation of the visual cycle: retinol dehydrogenase and retinol fluorescence measurements in vertebrate retina. Adv Exp Med Biol 533:353-60
Wang, Peng; Sun, Shu-Hui; Silver, Phyllis B et al. (2003) Methimazole protects from experimental autoimmune uveitis (EAU) by inhibiting antigen presenting cell function and reducing antigen priming. J Leukoc Biol 73:57-64

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